RhoA基因在胰腺癌组织中的表达及其与肿瘤临床病理特征的关系

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Abstract Objective: To investigate the expression of RhoA gene in tissues of pancreatic cancer and to discuss its significance during carcinogenesis and progression of tumor. Methods: Intratumor RhoA expression level was determined and compared with that in adjacent nontumorous pancreatic tissues using quantitative real time polymerase chain reaction and Western blot in 48 samples of pancreatic carcinoma. Results: The mRNA levels of RhoA were significantly higher in tumor tissues than that in the unaffected portions (t=3.669, P=0.0007). The expression of RhoA mRNA in the primary lesion was higher in patients with low and middle differentiation cancer (t=2.447, P=0.006), protal vein invasion (t=2.544, P=0.008), lymph node metastasis (t=2.351, P=0.041), and advanced pTNM stage (stage III/IV, t=2.667, P=0.025) than in those without. There was a significant difference between high RhoA protein levels in the tumor tissues and noncancerous tissues in pancreatic carcinoma patients(t=3.461, P=0.0003), the quantity of RhoA expression associated with tumor invasion and metastasis. There was a significant association between high tumor RhoA protein levels and low and middle differentiation cancer (t=2.323, P=0.016), and the presence of protal vein invasion (t=2.119, P=0.036), lymph node metastasis(t=2.117, P=0.029), and advanced pTNM stage (t=2.776, P=0.004). Conclusion: There is a significant correlation among RhoA expression, tumor stage, and metastasis. The expression of RhoA can be used as a good tumor marker for invasive and advanced pancreatic carcinoma as well as a prognosis predictor.

Key words： pancreatic neoplasms pathology, clinical gene expression RhoA

Received: 27 April 2013

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	LI Jinhai
	DAI Huawei
	ZHANG Haifeng.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I2/130

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