肠三叶因子通过自噬在急性移植物抗宿主病中发挥保护作用

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Abstract Objective: To observe the protection of ITF in acute graft versus host disease (aGVHD) models, and to investigate the role of autophagy in the protective effect of ITF in aGVHD. Methods: aGVHD model was established from donor mice C57BL/6 (H-2b) to host mice BALB/C (H-2d). According to different treatment, mice were divided into 4 groups. No any treatment in control group, BM group only reveiced bone marrow transplantation, while aGVHD group received a combination of bone marrow and spleen cells, ITF group accepted prophylaxis ITF treatment on basis of aGVHD group treatment. The aGVHD level was evaluated by clinical scores, survival time, peripheral leukocyte counts and expression of LC3 by immunohistochemical staining. Results: The model of aGVHD was successfully established. Compared with ITF group, aGVHD group performanced earlier aGVHD symptoms, shorter survial time, lower peripheral WBC counts and higher aGVHD clinical scores (P<0.05). Immunohistochemical staining (IHC) showed that aGVHD group had higher expression of LC3 compared with BM group and ITF group (P<0.05). Conclusion: ITF play a protective role in aGVHD, which is associated with decrease of autophagy.

Key words： acute graft versus host disease intestinal trefoil factor autophagy allogenic bone marrow transplantation mice

Received: 16 March 2017

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	CHEN Huiyao
	ZHANG Wei
	FANG Fang
	HE Ying
	ZHUANG Qiang
	JIANG Songfu

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I7/480

[1] CHAO N. Graft-verses-host disease: the view point from donor T cell[J]. Biol Blood Marrow Transplat, 1997, 3(1): 1-10.
[2] FERRARA J L, REDDY P. Pathophysiology of graft-versus-host disease[J]. Semin Hematol, 2006, 43(1): 3-10.
[3] 蔡芳芳. 肠三叶因子在小鼠肠道急性移植物抗宿主病中的表达及其作用[D]. 温州: 温州医学院, 2009.
[4] LEVINE B, MIZUSHIMA N, VIRGIN H W. Autophagy in immunity and inflammation[J]. Nature, 2011, 469(7330): 323-335.
[5] BENJAMIN J L, SUMPTER R, LEVINE B, et al. Intestinal epithelial autophagy is essential for host defense against invasive bacteria[J]. Cell Host Microbe, 2013, 13(6): 723-734.
[6] COOKE K R, KOBZIK L, MARTIN T R, et al. An experimental model of idiopathic pneumonia syndrome after bone marrow transplantation: I. The roles of minor h antigens and endotoxin[J]. Blood, 1996, 88(8): 3230-3239.
[7] SOSOW R A, DANNENBERG A J, RUSH D, et al. Cox-2 is expressed in human pulmonary, colonic, and mammary tumors[J]. Cancer, 2000, 89(12): 2637-2645.
[8] TAUPIN D, PODOLSKY D K. Trefoil factors: initiators of mucosal healing[J]. Nat Rev Mol Cell Biol, 2003, 4(9): 721-732.
[9] DIGNASS A, LYNCH-DEVANEY K, KINDON H, et al.Trefoil peptides promote epithelial migration through a transforming growth factor beta-independent pathway[J]. J Clin Invest, 1994, 94 (1): 376.
[10] 苏华芳, 邹阮敏, 俞康, 等. 肠三叶因子对甲氨蝶呤所致肠黏膜细胞损伤的保护作用[J]. 中华消化杂志, 2011, 31(3):164-168.
[11] DERETIC V, LEVINE B. Autophagy, immunity, and microbial adaptations[J]. Cell Host Microbe, 2009, 5(6): 527-549.
[12] HARRIS J. Autophagy and cytokines[J]. Cytokine, 2011, 56 (2): 140-144.
[13] LEE H K, MATTEI L M, STEINBERG B E, et al. In vivo requirement for Atg5 in antigen presentation by dendritic cells[J]. Immunity, 2010, 32(2): 227-239.
[14] MORRRIS S, SWANSON M S, LIEBERMAN A, et al. Autophagy-mediated dendritic cell activation is essential for innate cytokine production and apc function with respiratory syncytial virus responses[J]. J Immunol, 2011, 187(8): 3953-3961.
[15] PUA H H, GUO J, KOMATSU M, et al. Autophagy is essential for mitochondrial clearance in mature T lymphocytes[J]. J Immunol, 2009, 182(7): 4046-4055.
[16] TANIDA I, UENO T E. LC3 conjugation system in mammalian autophagy[J]. Int J Biochem Cell Biol, 2004, 36(12): 2503-2518.