噬菌体展示技术筛选卵巢癌细胞表面转移相关分子结合肽

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Abstract Objective: Using 7-mer phage display peptide library in vitro to isolate metastasis related peptides on the surface of the high metastatic potential ovarian tumor cell line HO-8910PM. Methods: Phage display technology with biopanning and rapid analysis of selective interactive ligands between cell lines HO-8910 and HO-8910PM was utilized. After five rounds of biopanning, 14 phage clones were randomly selected for sequencing of DNA. Using ELISA and phage competitive binding experiment the affinity of positive phages combined with HO-8910PM cells was identified. Results: The phage clone named Z3 (peptide LRLRNTR) showed higher affinity to HO-8910PM cells. Conclusion: The peptide LRLRNTR screened by phage display technology may offer a new direction for early diagnosis, metastasis and treatment of ovarian tumor.

Key words： ovarian tumor phage display technology phage peptide library peptides

Received: 15 October 2013

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	LI Wenju
	WANG Ledan
	LV Jieqiang.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I2/127

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