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| Paeoniflorin up-regulates expression of Sirt1 in spinal dorsal horn and dorsal root ganglia in chronic constriction injury rat |
| ZHENG Hui1, YANG Liuying1, Zheng Wen1,2, ZHANG Juan1, GUO Xiaowen1, TAO Tao1. |
1.Department of Anesthesia, the First Affiliated Hospital of Zhejiang Chinese Medical University,
Hangzhou, 310000; 2.The First Clinical Medical College, Wenzhou Medical University, Wenzhou, 325035 |
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| Cite this article: |
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ZHENG Hui,YANG Liuying,Zheng Wen, et al. Paeoniflorin up-regulates expression of Sirt1 in spinal dorsal horn and dorsal root ganglia in chronic constriction injury rat[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(6): 435-440.
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Abstract Objective: To observe the effect of paeoniflorin on pain behavior and the expression of Sirt1 in spinal dorsal horn and dorsal root ganglion in rats with chronic constriction injury. Methods: Forty-eight male SD rats were randomly divided into sham operation group, negative contract group (SC group), CCI group and paeoniflorin group. Heat shrinkable foot reflex latency (TWL) and mechanical paw withdrawal reflex threshold (MWT) were detected for rats in each group at 1 day before operation and postoperative 3, 7 and 14 days. Rats were sacrificed at 3, 7 and 14 days after surgery then L4-5 segment of spinal cord and dorsal root section organization was taken, and real-time PCR was used for expression of Sirt1, Western blot was used in the same time to detect the change of Sirt1 protein level. Results: At 3, 7 and 14 days after surgery, TWL and MWT of CCI group decreased significantly compared with sham group (P<0.05), and achieved the minimum value at the third day. Real-time PCR showed that the expression of Sirt1 of CCI group of spinal dorsal horn and dorsal root ganglia were significantly decreased at the 7th day and 14th day after surgery compared with sham group (P<0.05); the expression of Sirt1 of paeoniflorin group was upregulated compared with SC group (P<0.05); the results of Western blot also showed that the level of Sirt1 protein in the dorsal horn and dorsal root ganglion were gradually increased with time, and there was difference between the Sirt1 group and the SC group at 14th day. Conclusion: Paeoniflorin can alleviate by pain neuropathic disease caused, and the up regulation of the expression of Sirt1 protein in dorsal horn and dorsal root ganglion of the spinal cord is a possible mechanism.
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Received: 05 August 2016
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