|
|
|
| The effects of Evodiamine on the proliferation and apoptosis of pancreatic cancer SW1990 cells in vivo and in vitro |
| CHEN Hui, WANG Zhaohong, CHEN Long, FAN Hengwei, ZHOU Bin, XU Lubai, TONG Hongfei. |
| Department of Hepatobiliary, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027 |
|
| Cite this article: |
|
CHEN Hui,WANG Zhaohong,CHEN Long, et al. The effects of Evodiamine on the proliferation and apoptosis of pancreatic cancer SW1990 cells in vivo and in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(6): 431-434,440.
|
|
|
|
Abstract Objective: To investigate the effects of Evodiamine on the proliferation and apoptosis of pancreatic cancer SW1990 cells. Methods: Pancreatic cancer SW1990 cells were mixed with different concentrations of Evodiamine (0, 5, 10, 20, 40, 80 μmol/L) after 12, 24, 48 hours respectively. The SW1990 cells’ proliferation was detected by cell counting Kit-8 (CCK-8). The SW1990 cells’ cycle was detected with flow cytometry test. Nude mouse models were established with orthotopic transplantation tumor, which were injected with different concentrations of Evodiamine for 2 weeks. The orthotopic transplantation tumor’s weight was measured after sacrificed. The expression of Active Caspase-3 and Active Caspase-8 in tumor tissues was detected by Western blot and immunohistochemistry. Results: Evodiamine inhibited the proliferation effect of SW1990 cells by concentration and time dependence. The quantity of pancreatic cancer SW1990 cells in G2/M phase significantly increased. The expressions of Active Caspase-3 and Active Caspase-8 protein significantly increased. With increasing of Evodiamine concentrations, the inhibiting effect on the nude mouse’s growth of pancreas transplantation tumor was more remarkable and the expressions of Active Caspase-3 and Active Caspase-8 increased much more obviously in the tumor tissues. Conclusion: Evodiamine can significantly inhibit human pancreatic cancer SW1990 cells’ proliferation with concentration and time dependence. Its mechanism may retard the growth of pancreatic cancer cells in G2/M phase. In the meantime, Evodiamine can activate Caspase-8 and Caspase-3, then promote the apoptosis of pancreatic cancer cells.
|
|
Received: 13 October 2016
|
| |
|
|
|
[1] STATHIS A, MOORE M J. Advanced pancreatic carcinoma: current treatment and future challenges[J]. Nat Rev Clin Oncol, 2010, 7(3): 163-172.
[2] TEAGUE A, LIM K H, WANGGILLAM A. Advanced pancreatic adenocarcinoma: a review of current treatment strategies and developing therapies[J]. Ther Adv Med Oncol,2015, 7(2): 68-84.
[3] SU J, ZHOU X, WANG L, et al. Curcumin inhibits cell growth and invasion and induces apoptosis through down-regulation of Skp2 in pancreatic cancer cells[J]. Am J Cancer Res, 2016, 6(9): 1949-1962.
[4] MUJAHID Y, SANJEEV B, MOMIN M, et al. Synthesis, characterization and anti-tumor activity of novel thymoquinone analogs against pancreatic cancer[J]. Bioorg Med Chem Lett, 2013, 23(10): 3101-3104.
[5] HAN S, WOO J K, JUNG Y, et al. Evodiamine selectively targets cancer stem-like cells through the p53-p21-Rb pathway[J]. Biochem Biophys Res Commun, 2016, 469(4): 1153-1158.
[6] JIANG J L, HU C P. Evodiamine: a novel anti-cancer alkaloid from Evodia rutaecarpa[J]. Molecules, 2009, 14(5): 1852-1859.
[7] WEI W T, CHEN H, WANG Z H, et al. Enhanced antitumor efficacy of gemcitabine by evodiamine on pancreatic cancer via regulating PI3K/Akt pathway[J]. Int J Bio Sci, 2012, 8(1): 1-14.
[8] JIA Y, DU H, YAO M, et al. Chinese herbal medicine for myelosuppression induced by chemotherapy or radiotherapy: a systematic review of randomized controlled trials[J]. Evid Based Complement Alternat Med, 2015, 2015(3):690976.
[9] MAN S, GAO W, WEI C, et al. Anticancer drugs from traditional toxic Chinese medicines[J]. Phytother Res, 2012, 26(10): 1449-1465.
[10] 张醇, 梁华平. 吴茱萸碱抗肿瘤活性研究进展[J]. 中国新药杂志, 2010(17): 1558-1562.
[11] 张元新, 葛雅琨. 吴茱萸碱与紫杉醇协同抑制胃癌细胞SGC-7901的研究[J]. 吉林农业大学学报, 2014(3): 314-318. |
|
|
|
