小鼠暴露式气管注射给药方式探索及对照组设置

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Abstract Objective: To make water-soluble drugs instilled through the exposed trachea a stable and roughly equal distribution in the lungs of mice and to design the control group scientifically. Methods: Intervention measure, mouse position, instillment speed, minimum volume that requires and maximum volume that tolerates were all taken into consideration to make the distribution of the drug (Methlene Blue instead) in lung lobes in mice (weighting from 18 g to 30 g) stable and roughly equal. Then taking lipopolysaccharide (LPS) as example, the LPS group and normal saline (NS) group were treated with LPS (2 mg/mL) and normal saline (NS) respectively in the way above mentioned in mice weighting from 20 g to 24 g. The normal group was with no pretreatment. After modeling for 3 h, 8 h, 14 h, the level of monocyte chemoattractant protein-1 (MCP-1) in bronchoalveolar lavage fluid (BALF) detected by enzyme-linked immunoabsorbent assay (ELISA), the polymorphonuclear neutrophil percentage (PMN%) in BALF tested with Wright-Giemsa stain, histopathological changes assayed by HE staining, the expression of intercellular adhesion molecule 1 (ICAM-1) assayed by immunohistochemical staining and the lung wet weight/dry weight ratio (W/D ratio) in lung tissue would be tested. Results: Using the process developed above, the distribution of Methlene Blue in lung lobes was stable and roughly equal. Compared with the normal group, the level of MCP-1 in BALF in the LPS group increased at 3 h, 8 h, and as time went by, the PMN% in BALF increased and histopathological changes appeared obvious, and both the expression level of ICAM-1 and the W/D ratio increased (P<0.05) in LPS group. Compared with the normal group, only the level of MCP-1 in BALF and the W/D ratio increased significantly (P<0.05) in the NS group at 3 h, and all measurements had no difference at 8 h and 14 h. Conclusion: The process developed here to instill water-soluble drugs through exposed trachea is successful. The control group should be treated with NS for the same time if researching the lung changes within 8 h, or just the normal mice instead if at least 8 hours.

Key words： mice intratracheal instillation water-soluble drug control group

Received: 02 October 2016

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	WANG Nujing
	PENG Chuanpeng
	ZHENG Shuang
	ZHOU Lingping
	XU Honglei

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I6/401

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