胰岛素样生长因子结合蛋白7对胃癌细胞株SGC-7901细胞周期的影响

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1367 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the effect of insulin-like growth factor binding protein 7 on gastric cancer SGC-7901 cells and its mechanisms. Methods: The recombinant expressing plasmid pCMV-XL4-IGFBP7 was constructed and transfected into SGC-7901 cells. Empty plasmid transfection was regarded the control. Cell proliferation was analyzed by MTS assay; cell apoptosis was detected by flow cytometer. Results: Compared with control group, the rate of SGC-7901 cell of pCMV-XL4-IGFBP7 group on G1 level was higher; while the rate of SGC-7901 cell of pCMV-XL4-IGFBP7 group on G2/M was lower than the control group. The proliferation of SGC-7901 cells had no significant change compared with the control group. Conclusion: IGFBP7 may inhibit gastric cancer SGC-7901 cells through influencing the cell cycle.

Key words： insulin-like growth factor binding protein-7 gastric tumor cell proliferation cell cycle

Received: 30 September 2016

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HU Jianhao
	CHEN Weiyan
	WANG Xiufang
	WANG Fenfen

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I4/296

[1] 何立丽, 孙桂芝, 张培影. 胃癌的病因病机研究进展[J]. 北京中医药, 2009, 28(3): 234-236.
[2] LIN Y, UEDA J, KIKUCHI S, et al. Comparative epidemiology of gastric cancer between Japan and China[J]. World Gastroenterol, 2011, 17(39): 4421-4428.
[3] YOON H, KIM N. Diagnosis and management of high risk group for gastric cancer[J]. Gut Liver, 2015, 9(1): 5-17.
[4] 中华人民共和国卫生部. 中国癌症预防与控制规划纲要(2004-2010)[M]. 中国肿瘤, 2004, 13(2): 65-68.
[5] SUSAN E, ERDMAN, THEOFILOS P. Cancer inflammation and regulatory T cells[J]. Int J Cancer, 2010, 127(4):768-779.
[6] SHINAHARA T, OHSHIMA K, MURAYAMA H, et al. Apoptosis and proliferation in gastric carcinoma: the association ion with histological type[J]. Histopathology, 1996, 29(2): 123-129.
[7] AKIELM M, RAJA SAKARAN D, GREDLER R, et al. Emerging role of insulin-like growth factor-binding protein 7 in hepatocellular carcinoma[J]. J Hepatocell Carcinoma, 2014, 1: 9-19.
[8] CHERT Y, PACYNA G M, YE F, et al. Insulin-like growth factor binding protein-related proteinl (IGFBP-rPI) has potential tumour-suppressive activity in human lung cancer[J]. Pathol, 2007, 211(4): 431-438.
[9] BURGER A M, ZHANG X, HUA L, et al. Down-reglation of Tl A12/mac25, a novel insulin-like growth factor binding protein related gene, is associated with disease progression in breast carcinomas[J]. Oneogene, 1998, 16(19): 2459-2467.
[10] MENESES-ECHAVE J F, JIMENEZ E G, RIO-VALLE J S, et al. The insulin-like growth factor system is modulated by exercise in breast cancer survivors: a systematic review and meta-analysis[J]. BMC Cancer, 2016, 16(1): 682.
[11] 满孝蕊, 胡绍燕, 岑建, 等. 胰岛素样生长因子结合蛋白相关蛋白1基因在儿童急性白血病中的表达及临床意义[J]. 肿瘤, 2010, 30(1): 53-56.
[12] 赵丹瑜, 李霞, 钟捷, 等. 胰岛素样生长因子结合蛋白7基因在胃癌细胞中的表达及甲基化调控[J]. 上海交通大学学报(医学版), 2009, 29(9): 1602-1605.
[13] YAN P S, CAO L X, ZHANG B Z. Efficient purification of antiproliferative polysaccharides from Hypsizigus marmoreus with radial flow chromatography[J]. Biotechnol Prog, 2014, 30(4): 872-878.