CD117和Sox10在恶性黑色素瘤中的表达及意义

Abstract
Figure/Table
References (0)
Related Citation (10)

Download: PDF (2074 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To analyze the value of CD117, Sox10 expression and c-kit gene amplification in mucous, acral and non-acral melanoma and explore the relationship between it and clinical pathology. Methods: The gene levels of c-kit in mucosal, acral and non acral melanoma were detected by PCR, with pigmented nevus as control. The protein expressions of CD117, sox10, 1-100 and HMB45 were detected by immunohistochemistry. Results: The expression of c-kit gene from the three MM groups was higher than that of pigmented nevus (P<0.05). The immunohistochemical results demonstrated that the number of positive cells of CD117 from the three MM groups was marginally higher than that of pigmented nevus (P<0.05). The number of positive cells of Sox10 in the three MM groups was higher than that of S-100 and HMB45 (P<0.05). The positive rate of CD117 in mucosal, acral and non acral MM was 78.5%, 73.6% and 73.3% respectively, while 25% in pigmented nevus control (P<0.05). The positive rate of Sox10, S-100 and HMB45 in MM was 100.0%, 87.5% and 70.8% respectively (P<0.05). Conclusion: C-kit gene amplification and its downstream transcription protein CD117 enhanced positive expression can be applied as clinical potential therapy target. The positive rate of Sox10 is higher than S-100 and HMB45, and may be applied as tumor differentiation marker for origins of melanocytes.

Key words： melanoma CD117 c-kit Sox10 immunohistochemistry

Received: 18 September 2016

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WEN Lihong
	LIN Ruipo
	CHEN Ade
	HUANG Zhaolang
	ZHENG Xiangyan
	LI Yangyang
	HUANG Kate
	CHEN Guorong

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I3/197

[1] 陈文静. 新疆地区恶性黑素瘤c-kit基因突变及CD117表达分析[D]. 乌鲁木齐: 新疆医科大学, 2013.
[2] 倪淑娟, 陈晓晨, 王奇峰, 等. 肛管直肠恶性黑色素瘤c-kit基因突变研究[C]//中华医学会病理学分会2010年学术年会, 2010.
[3] 胡孟钧, 魏建丽. 粘膜原发性恶性黑色素瘤10例病理分析[J]. 温州医学院学报, 1999, 29(3): 253.
[4] CSCO黑色素瘤专家委员会. 中国黑色素瘤诊治指南(2011版)[J]. 临床肿瘤学杂志, 2012, 17(2): 159-171.
[5] GARBE C, PERIS K, HAUSCHILD A, et al. Diagnosis and treatment of melanoma. European consensus-based interdisciplinary guideline-Update 2016[J]. Eur J Cancer, 2016, 63:201-217.
[6] 李腾政, 罗文, 张吉翔. SOX10在黑色素瘤中的作用[J]. 实用医学杂志, 2015, 1(19): 3267-3268.
[7] WEHRLE-HALLER B. The role of Kit-ligand in melanocyte development and epidermal homeostasis[J]. Pigment Cell Res, 2003, 16(3): 287-296.
[8] GIEHL K A, NAGELE U, VOLKENANDT M, et al. Protein expression of melanocyte growth factors (bFGF, SCF) and their receptors (FGFR-1, c-kit) in nevi and melanoma[J]. J Cutan Pathol, 2007, 34(1): 7-14.
[9] CRONIN J C, WATKINS-CHOW D E, INCAO A, et al. SOX10 ablation arrests cell cycle, induces senescence, and suppresses melanomagenesis[J]. Cancer Res, 2013, 73(18): 5709-5718.
[10] AOKI Y, SAINT-GERMAIN N, GYDA M, et al. Sox10 regulates the development of neural crest-derived melanocytes in Xenopus [J]. Dev Biol, 2003, 259(1): 19-33.
[11] LIU H G, KONG M X, YAO Q, et al. Expression of Sox10 and c-kit in sinonasal mucosal melanomas arising in the Chinese population[J]. Head Neck Pathol, 2012, 6(4): 401-408.
[12] NONAKA D, CHIRIBOGA L, RUBIN B P. Sox10: a panschwannian and melanocytic marker[J]. Am J Surg Pathol, 2008, 32(9): 1291-1298.
[13] DZWIERZYNSKI W W. Managing malignant melanoma[J].Plast Reconstr Surg, 2013, 132(3): 446-460.
[14] SATZGER I, SCHAEFER T, KUETTLER U, et al. Analysis of c-kit expression and KIT gene mutation in human mucosal melanomas[J]. Br J Cancer, 2008, 99(12): 2065-2069.
[15] POSCH C, MOSLEHI H, SANLORENZO M, et al. Pharmacological inhibitors of c-kit block mutant c-kit mediated migration of melanocytes and melanoma cells in vitro and in vivo[J]. Oncotarget, 2016, 7(29): 45916-45925.
[16] KELSCH R N. Sorting out SOX10 functions in neural crest development[J]. Bio Essays, 2006, 28(8): 788-798.