卵巢去细胞生物支架的制备及鉴定

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (2164 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To prepare decullularized biologic scaffolds of part of porcine ovarian tissue by soaking with rotating, and then indentify their characteristics. Methods: Thirty ovaries were excised from healthy immature pigs, and then cut transversely into pieces at the thickness of 6 mm uniformly. At room temperature, they were soaked into 1% SDS solutions and rotated on a tablet oscillator at the speed of 120 r/min for 36 h. At last they were washed with deionized water to get rid of residual SDS and DNA fragments. After decellularization, histochemical staining was done, the residual DNA was quantitative determined, examined under scanning electron microscope examination and did immunofluorescence to observe the removal of cells, the three-dimensional structure of the scaffolds, and the reservation of collagens and proteins in the extracellular matrix. Results: Quantitative analysis showed that only 15.4% DNA remained in the decellularized scaffolds compared to the normal control tissues; HE straining, scanning electron microscope and immunofluorescence showed there were no cells left in the scaffolds, at the same time, the three-dimensional structure and the collagens in the extracellular matrix kept intact. Conclusion: The method of soaking with rotating can remove all cells from the ovarian tissue, maintain the supporting structure of extracellular matrix relatively completely, and reserve collagens and proteins at a certain degree.

Key words： decellularization ovary tissue scaffolds extracellular matrix ovarian follicle

Received: 01 November 2016

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	LAN Li
	WANG Yan
	FU Yanghua
	LIU Shuailiang
	LI Ting
	WANG Zhibin
	XI Haitao
	YANG Yunjun
	MEI Jin.

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I3/182

[1] FENTON A J. Premature ovarian insufficiency: Pathogenesis and management[J]. J Midlife Health, 2015, 6(4): 147-153.
[2] 杨念念, 严亚琼, 龚洁, 等. 中国2003~2007年卵巢癌发病与死亡分析[J]. 中国肿瘤, 2012, 21(6): 401-405.
[3] PÉREZ-ANDÚJAR A, NEWHAUSER W D, TADDEI P J, et al. The predicted relative risk of premature ovarian failure for three radiotherapy modalities in a girl receiving craniospinal irradiation[J]. Phys Med Biol, 2013, 58(10): 3107-3123.
[4] MAYORGA J, ALPÍZAR-RODRÍGUEZ D, PRIETO-PADILLA J, et al. Prevalence of premature ovarian failure in patients with systemic lupus erythematosus[J]. Lupus, 2016,25(7): 675-683.
[5] MEATTINI I, SAIEVA C, MEACCI F, et al. Impact of ageon cytotoxic-induced ovarian failure in breast cancer treated with adjuvant chemotherapy and triptorelin[J]. Future Oncol, 2016, 12(5): 625-635.
[6] JORDAN S J, WILSON L F, NAGLE C M, et al. Cancers in Australia in 2010 attributable to and prevented by the use of menopausal hormone therapy[J]. Aust N Z J Public Health, 2015, 39(5): 434-440.
[7] LARONDA M M, JAKUS A E, WHELAN K A, et al. Initiation of puberty in mice following decellularized ovary transplant[J]. Biomaterials, 2015, 50: 20-29.
[8] OTT H C, MATTHIESEN T S, GOH S K, et al. Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart[J]. Nat Med, 2008, 14(2): 213-221.
[9] BADYLAK S F, GILBERT T W. Immune response to biologic scaffold materials[J]. Semin Immunol, 2008, 20(2): 109-116.
[10] 张建色, 王辉, 邵培刚, 等. 去细胞全肾生物支架的制备与鉴定[J]. 解剖学报, 2012, 43(2): 253-257.
[11] MAZZA G, ROMBOUTS K, RENNIE H A, et al. Decellularized human liver as a natural 3D-scaffold for liver bioengineering and transplantation[J]. Sci Rep, 2015, 5: 13079.
[12] 陈婵, 王志斌, 翁杰, 等. 全肺去细胞生物支架的制备与鉴定[J]. 温州医科大学学报, 2016, 46(2): 88-91.
[13] 林贤丰, 邵营宽, 王辉, 等. 离体大鼠胰腺去细胞生物支架的制备与鉴定[J]. 解剖学报, 2012, 43(5): 717-722.
[14] 彭蒙蒙, 林贤丰, 邵培刚, 等. 灌注法制备离体大鼠心脏脱细胞基质[J]. 解剖学报, 2012, 43(4): 559-563.
[15] JIANG T, REN X J, TANG J L, et al. Preparation and characterization of genipin-crosslinked rat acellular spinal cord scaffolds[J]. Mater Sci Eng C Mater Biol Appl, 2013, 33(6): 3514-3521.
[16] SOLEIMANI R, HEYTENS E, OKTAY K. Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants[J]. PLoS One, 2011, 6(4): e19475.
[17] CRAPO P M, GILBERT T W, BADYLAK S F. An overview of tissue and whole organ decellularization processes[J].Biomaterials, 2011, 32(12): 3233-3243.
[18] WANG X, CUI J, ZHANG B Q, et al. Decellularized liver scaffolds effectively support the proliferation and differentiation of mouse fetal hepatic progenitors[J]. J Biomed Mater Res A, 2014, 102(4): 1017-1025.
[19] LU X, LI W, FUKUMOTO S, et al. The ameloblastin extracellular matrix molecule enhances bone fracture resistance and promotes rapid bone fracture healing[J]. Matrix Biol, 2016, 52-54: 113-126.
[20] LI H, JIANG W, REN W, et al. Downregulation of the Yesassociated protein is associated with extracellular matrix disorders in ascending aortic aneurysms[J]. Stem Cells Int, 2016, 2016: 6786184.
[21] JIANG W C, CHENG Y H, YEN M H, et al. Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering[J]. Biomaterials, 2014, 35(11): 3607-3617.
[22] LECHT S, STABLER C T, RYLANDER A L, et al. Enhanced reseeding of decellularized rodent lungs with mouse embryonic stem cells[J]. Biomaterials, 2014, 35(10): 3252-3262.
[23] YU Y L, SHAO Y K, DING Y Q, et al. Decellularized kidney scaffold-mediated renal regeneration[J]. Biomaterials, 2014, 35(25): 6822-6828.
[24] ZHANG J, WANG Z, LIN K, et al. In vivo regeneration of renal vessels post whole decellularized kidneys transplantation[J]. Oncotarget, 2015, 6(38): 40433-40442.
[25] MEI J, YU Y, LI M, et al. The angiogenesis in decellularized scaffold-mediated the renal regeneration[J]. Oncotarget, 2016, 7(19): 27085-27093.
[26] LÜ X, ZHAI W, ZHOU Y, et al. Crosslinking effect of Nordihydroguaiaretic acid (NDGA) on decellularized heart valve scaffold for tissue engineering[J]. J Mater Sci Mater Med, 2010, 21(2): 473-480.

[1]	. [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(9): 0-.