长链脂肪乳剂逆转布比卡因导致的心肌细胞膜流动性降低

Abstract
Figure/Table
References (0)
Related Citation (9)

Download: PDF (1766 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To study the effects of long-chain lipid emulsion on the changes of membrane fluidity induced by bupivacaine in H9c2 cardiomyocyte. Methods: By the combination of confocal laser scanning microscope and fluorescence recovery after photobleaching, the effects of long-chain lipid emulsion and bupivacaine on the membrane fluidity in H9c2 cardiomyocyte, whose membrane phospholipid was labelled with NBD-C6-HPC specific fluorescent probes, were determined. Results: Comparing with the Con group, the LCT group showed no significant difference in membrane fluidity. The Bu-1000 group showed a decreased recovery ratio when compared with the Con group (P<0.01), LCT group (P<0.01) and Bu-100 group (P<0.01), and a decreased diffusion coefficient when compared with the Con group (P<0.05) and the LCT group (P<0.01). Compared with Bu-1000 group, the Bu-1000+LCT group showed an increased recovery ratio (P<0.05) and an increased diffusion coefficient (P<0.01). Compared with Bu-100 group, the Bu-100+LCT group showed an increased diffusion coefficient (P<0.01). Conclusion: Long-chain lipid emulsion can reverse the decrease of membrane fluidity induced by bupivacaine in H9c2 cardiomyocyte.

Key words： lipid emulsions bupivacaine fluorescence recovery after photobleaching membrane fluidity recovery ratio diffusion coefficient

Received: 11 April 2016

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHEN Zhe
	ZHAO Shishi
	LIN Tingting
	LIU Qiaoyan
	DONG Jiaojiao
	XU Xuzhong.

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I3/178

[1] WEINBERG G L, VADEBONCOUER T, RAMARAJU G A, et al. Pretreatment or resuscitation with a lipid infusion shifts the dose-response to bupivacaine-induced asystole in rats[J]. Anesthesiology, 1998, 88(4): 1071-1075.
[2] MAZOIT J X, LE GUEN R, BELOEIL H, et al. Binding of long-lasting local anesthetics to lipid emulsions[J]. Anesthesiology, 2009, 110(2): 380-386.
[3] SILVEIRA L, HIRABARA S M, ALBERICI L C, et al. Effect of lipid infusion on metabolism and force of rat skeletal muscles during intense contractions[J]. Cell Physiol Biochem, 2007, 20(1-4): 213-226.
[4] CHEN H, XIA Y, ZHU B, et al. Measurement of the efficacy of 2% lipid in reversing bupivacaine- induced asystole in isolated rat hearts[J]. BMC Anesthesiol, 2014, 14: 60.
[5] TSUCHIYA H, MIZOGAMI M, TAKAKURA K. Reversedphase liquid chromatographic retention and membrane activity relationships of local anesthetics[J]. J Chromatogr A,2005, 1073(1-2): 303-308.
[6] 张志毅, 周涛, 郭姗姗, 等. 应用FRAP方法计算细胞膜脂流动性[J]. 现代科学仪器, 2007(6): 68-71.
[7] AXELROD D, KOPPEL D E, SCHLESSINGER J, et al. Mobility measurement by analysis of fluorescence photo-bleaching recovery kinetics[J]. Biophys J, 1976, 16(9): 1055-1069.
[8] 汶文瑾, 刘昌华, 李晓艳, 等. 动脉粥样硬化中单核细胞膜流动性研究[J]. 激光生物学报, 2014, 23(2): 122-126.
[9] SCHOOTEMEIJER A, GORTER G, TERTOOLEN L G, et al. Relation between membrane fluidity and signal transduction in the human megakaryoblastic cell line MEG-01[J]. Biochim Biophys Acta, 1995, 1236(1): 128-134.
[10] 李喜艳, 王加启, 卜登攀, 等. 多不饱和脂肪酸对细胞膜功能影响的研究进展[J]. 生物技术通报, 2009(12): 22-26.
[11] 李永荣, 郭贵林, 姚德厚, 等. 利多卡因对兔内毒素血症肺细胞膜磷脂酶A2及膜流动性的影响[J]. 中华麻醉学杂志, 1998, 18(6): 343-345.
[12] NANO J L, NOBILI C, GIRARD-PIPAU F, et al. Effects of fatty acids on the growth of Caco-2 cells[J]. Prostaglandins Leukot Essent Fatty Acids, 2003, 69(4): 207-215.
[13] WANTEN G J, NABER A H. Human neutrophil membrane fluidity after exposure to structurally different lipid emulsions[J]. JPEN J Parenter Enteral Nutr, 2001, 25(6): 352-355.
[14] TSUCHIYA H, UENO T, MIZOGAMI M, et al. Local anesthetics structure-dependently interact with anionic phospholipid membranes to modify the fluidity[J]. Chem Biol Interact, 2010, 183(1): 19-24.