miR-34c对II型子宫内膜癌HEC-1-B细胞生长和凋亡的影响

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Abstract Objective: To investigate the effect miR-34c on the growth and apoptosis of type II endometrial carcinoma cells (HEC-1-B cell line). Methods: Up-regulated the expression of miR-34c by transfecting cells with hsa-miR-34c mimics, the transfection efficiency was detected by flow cytometry and further verified by quantitative real-time PCR (qRT-PCR). Cell proliferation assay by CCK-8 and colony formation assay applied to demonstrate that miR-34c could inhibit the growth of HEC-1-B cells. Cells apoptosis assay was analyzed by flow cytometry. Results: The transfection efficiency was 81.16%. The overexpression of miR-34c (detected by qRT-PCR) could inhibit cell proliferation, colony formation and promote cells apoptosis (P<0.05). Conclusion: MiR-34c can inhibit the growth and promote apoptosis of HEC-1-B cells significantly, and function is a potential tumor suppressor in type II endometrial carcinoma.

Key words： miR-34c type II endometrial carcinoma cell proliferation apoptosis

Received: 25 September 2013

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	LI Fuyao
	XUE Jisen
	HUANG Yibo
	CHEN Huijun
	ZHENG Feiyun

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I2/86

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