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| Stablishment of vascular endothelial cell injury model induced by high methionine feed diet in rats |
| REN Xiaoli1, Yang Bo2, CHEN Xiwen1, LOU Yongliang3. |
1.Laboratory Animal Research Center, Wenzhou Medical University, Whenzou, 325035; 2.School of Environment Science and Public Health, Wenzhou Medical University, Whenzou, 325035; 3.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Whenzou, 325035
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| Cite this article: |
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REN Xiaoli,Yang Bo,CHEN Xiwen, et al. Stablishment of vascular endothelial cell injury model induced by high methionine feed diet in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(1): 47-51.
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Abstract Objective: To investigate the effects of the diet rich in different proportions of methionine on endothelial injury in sprague-dawley (SD) rats. Methods: Male SD rats were randomly divided into the normal diet group (CR), 1% methionine loading diet group (M1 R), 3% methionine loading diet group (M3 R) and 5% methionine loading diet group (M5 R), and blood in tail vein were drawn at 0, 2nd, 4th, 6th and 8th week, respectively. After 8 weeks, serum concentrations of homocysteine (Hcy) were measured, and the endothelial ultrastructure changes in aorta wall were observed using scanning electron microscope. Results: A significant interaction between high methionine diet and feeding duration on endothelial injury was found (P<0.05), and serum levels of Hcy were significantly increased in rats fed with high methionine diet (P<0.05), with feeding duration increasing. Compared with CR, serum level of Hcy in M3 and M5 R was significantly higher at 2nd week (P<0.01), whereas serum Hcy in M1 R was significantly higher at 4th week (P<0.05). Endothelial cells in M1 R were imperfectly unanimous in size, and the cell surface shrinked. Endothelium in M3 R and M5 R were found to be damaged by worms kinds of harming typical, with enclose wall thrombus and the fat deposition. Conclusion: High dietary intake of methionine is significantly associated with endothelial injury, which suggests that a method setting up endothelial injury animal models would be established.
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Received: 08 April 2016
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