Wnt7b/β-catenin信号通路在大鼠哮喘气道重塑中的作用

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Abstract Objective: To explore the role of Wnt7b/β-catenin signaling pathway in airway remodeling in asthma. Methods: This study enrolled 16 8-week-old healthy male Sprague-Dawley (SD) rats (SPF grade), weighting from 180-200 g, without specific pathogens, and assigned them into asthma group and normal control group. Asthmatic airway remodeling models were established in rats with ovalbumin while the normal control group was given spray of physiological saline. HE staining was used to observe pulmonary pathological structure of two groups of rats, and color medical image analysis was used to determine the thickness of bronchial wall (Wat) and the thickness of smooth muscles (Wam). Immunohistochemical method was used to detect the distribution and protein expression of Wnt7b, β-catenin and c-Myc in the lung tissues, qRT-PCR was used to determine the mRNA expressions of Wnt7b, β-catenin and c-Myc. Results: Wat and Wam of asthma group were significant higher than those in normal group. The protein expression of Wnt7b, β-catenin, c-Myc detected by IHC in asthma group were significant higher than those in normal group (P<0.01, respectively). The mRNA expression of Wnt7b, β-catenin, c-Myc detected by qRT-PCR in asthma group were significant higher than those in normal group (P<0.01, respectively). There were significantly positive correlation between Wat and the protein expression of Wnt7b, β-catenin and c-Myc, also between Wam and the protein expression of Wnt7b, β-catenin and c-Myc. Conclusion: Wnt7b, β-catenin and c-Myc play important roles in airway remodeling in asthma.

Key words： asthma airway remodeling Wnt signal pathway β-catenin rats

Received: 31 March 2016

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	ZHU Tingting
	WENG Cuiye
	JIA Xiaoxiao
	ZENG Zeyu
	WANG Lei
	LI Changchong
	ZHANG Weixi.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I1/14

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