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| Dynamic changes of TNF-α and gelatinase on murine viral myocarditis model |
| ZHOU Aihua1, YUE Yayan2, JIN Zengyou1, PAN Lulu3, ZHANG Yingying1, WANG Dan1, QIAN Yan1, ZHANG Yuanhai3, WU Rongzhou3, CHU Maoping3. |
1.Department of Pediatrics, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Pediatric Cardiovascular disease, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325027
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| Cite this article: |
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RUAN Miaohua,WANG Dan,WANG Kai, et al. Dynamic changes of TNF-α and gelatinase on murine viral myocarditis model[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(12): 896-900.
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Abstract Objective: To explore the dynarnic changes and its meaning of TNF-α and gelatinase on the murine viral myocarditis model. Methods: Six-week-old inbred male mice were randomly assigned to control (n=35) and myocarditis group (n=60). The myocarditis and the control groups were inoculated intraperitoneally with 0.1 mL 10-5.69 TCID50/mL CVB3 or vehicle (PBS) alone respectively. Eight mice were sacrificed at 4th day, 10th day, 21th day and 30th day after injection. The serum content of TNF-α was measured by ELISA. The myocardial levels of MMP-2 and MMP-9 were determined by Western blot, MMP-2 mRNA and MMP-9 mRNA were measured by RT-PCR. Results: Compared with the control group, the protein and mRNA expression of MMP-2 and MMP-9 in the myocardium and the serum contents of TNF-α were significantly increased in myocarditis group (P<0.05). Conclusion: The expression of TNF-α and its downstream production MMP-2 and MMP-9 are increased in mice with acute viral myocarditis, and it may be involved in the pathogenesis of viral myocarditis.
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Received: 18 April 2016
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