WNK1激酶对Maxi K通道的调节作用

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Abstract Objective: To investigate the adjusting effect of WNK1 kinase on Maxi K channel in HEK-293T cells. Methods: The DNA plasmid carrying WNK1 and Maxi K gene was transfected into the HEK-293T cells, to observe the distribution and total protein expression of Maxi K in the HEK-293T cells via immunoflu-orescence microscopy and Western blot. Results: Immunofluorescence microscopic study showed that Maxi K expression was markedly increased in experimental group compared with the control group. Western blotting analysis showed that total Maxi K protein level was markedly increased in the presence of experimental group 1 and experimental group 2 compared with the control group (P<0.01). The total Maxi K protein level was markedly increased in experimental group 2 compared with the experimental group 1 (P<0.05). Conclusion: WNK1 can upregulate total protein expression of Maxi K, and the effect is enhanced with the increasing amount of WNK1 DNA, showing that the effect was dose-dependent.

Key words： WNK1 Maxi K pseudohypoaldosteronism II hypertension

Received: 04 March 2016

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	SHI Zhen
	ZHOU Lina
	ZHANG Yiqian
	WANG Dexuan
	ZHUANG Jieqiu.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I12/869

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