不同动脉血采集方法对小鼠血气分析结果的影响

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1554 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To explore ideal arterial blood collecting method in order to monitor blood gas analysis. Methods: Ninety male Balb/c mice were randomly divided into three groups: carotid artery cannula group, celiac artery puncture group, and cardiac punture group. 0.5-1.0 mL arterial blood samples were collected from carotid artery, celiac artery and left ventricle. These samples were tested in a Radiometer ABL90 Flex blood gas analyzer (Radiometer, Denmark) at 37 ℃ for pH, PaO2, SaO2, hemoglobin concentration tHb, oxyhemoglobin O2Hb, base excess cBase, standard bicarbonate SBC, P50, carboxyhemoglobin (COHb), reduced hemoglobin (RHb), K+, Na+, Cl-, Ca2+, as well as lactate and glucose concentrations. Results: There was significant difference in success rate, either in operative incision length or collection time among the three groups. Compared with the carotid artery cannula and celiac artery puncture group respectively, the parameters of PO2, SaO2, O2Hb were decreased, while RHb was significantly increased in cardiac punture group (P<0.05). And cBase, tHb in cardiac punture group were sharply lower than those in celiac artery puncture group (P<0.05). Increased K+ concentration, decreased Na+, Cl- concentration were found in the celiac artery puncture group compared with other two groups (carotid artery cannula group and cardiac punture group) (P<0.05). The concentrations of lactic acid, glucose in celiac artery puncture group were higher than those in carotid artery cannula group (P<0.05). Conclusion: Carotid artery cannula is a good and feasible method for arterial blood collecting, by which blood gas analysis, electrolytes and energy metabolism can be detected more accurately.

Key words： carotid artery celiac artery heart blood gas analysis mice

Received: 31 May 2016

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	WU Peiliang
	WANG Liangxing
	HUANG Xiaoying.

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I11/793

[1] BALDISSERA M D, VAUCHER R A, OLIVEIRA C B, et al. Blood gas analyses and other components involved in the acid-base metabolism of rats infected by Trypanosoma evansi [J]. J Adv Res, 2015, 6(6):1079-1082.
[2] SCHREIER D, HACKER T, SONG G, et al. The role of collagen synthesis in ventricular and vascular adaptation to hypoxic pulmonary hypertension[J]. J Biomech Eng, 2013, 135(2): 0210181-0210187.
[3] ZHANG Y, TALWAR A, TSANG D, et al. Macrophage migration inhibitory factor mediates hypoxia-induced pulmonary hypertension[J]. Mol Med, 2012, 18(1): 215-223.
[4] VLAAR A P, WOLTHUIS E K, HOFSTRA J J, et al. Mechanical ventilation aggravates transfusion-related acute lung injury induced by MHC-I class antibodies[J]. Intensive Care Med, 2010, 36(5): 879-887.
[5] DALKARA T, IRIKURA K, HUANG Z, et al. Cerebrovascular responses under controlled and monitored physiological conditions in the anesthetized mouse[J]. J Cereb Blood Flow Metab, 1995, 15(4): 631-638.
[6] HUPPE T, LORENZ D, MAURER F, et al. Exhalation of volatile organic compounds during hemorrhagic shock and reperfusion in rats: an exploratory trial[J]. J Breath Res, 2016, 10(1): 016016.
[7] 周仁鸥, 王劲, 刘江伟. 沙漠干热环境中暑大鼠的血气分析变化特点[J]. 中华危重病急救医学, 2015, 27(8): 653-657.
[8] CARGNONI A, PICCINELLI E C, RESSEL L, et al. Conditioned medium from amniotic membrane-derived cells prevents lung fibrosis and preserves blood gas exchanges in bleomycin-injured mice-specificity of the effects and insights into possible mechanisms[J]. Cytotherapy, 2014, 16(1): 17-32.
[9] 徐金中, 叶向红. 不同浓度肝素溶液对动脉血气分析结果
及凝血的影响[J]. 中国误诊学杂志, 2012, 12(3): 543.
[10] THAL S C, PLESNILA N. Non-invasive intraoperative
monitoring of blood pressure and arterial pCO2 during sur-
gical anesthesia in mice[J]. J Neurosci Methods, 2007, 159(2): 261-267.
[11] DERKACH K V, SHPAKOV A O, KUZNETSOVA L A, et al. The hormone-sensitive adenylyl cyclase system of the ciliate Dileptus anser[J]. Tsitologiia, 2002, 44(11): 1129-1133.
[12] WEEKERS F, GIULIETTI A P, MICHALAKI M, et al. Metabolic, endocrine, and immune effects of stress hyperglycemia in a rabbit model of prolonged critical illness[J]. Endocrinology, 2003, 144(12): 5329-5338.
[13] 殷健, 郭会彩, 王永利. 肾上腺素能受体对心肌钠钾泵的亚基特异性调节[J]. 中国药理学通报, 2011, 27(2): 156-158.
[14] 杨翠燕, 王金凤, 王芳, 等. 急性心肌梗死早期应激反应对血电解质的影响[J]. 中国实验诊断学, 2015, 19(5): 800-802.
[15] 林广玲, 黄林锋, 邱树彬, 等. 2型糖尿病与凝血功能障碍[J]. 中国血液流变学杂志, 2005, 15(1): 86-87, 94.