雪菊黄酮对脂肪变肝细胞的降脂效果

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Abstract Objective: To study the lipid-lowering effect of flavonoids from Coreopsis tinctoria on the liver cells with fatty degeneration. Methods: The medical fat emulsion injection was used as fat resource to make human liver fat cell model on normal liver cell line L-02. With different concentrations (20, 50, and 100 μmol/L
flavonoids from Coreopsis tinctoria were co-cultured with hepatocytes with fatty degeneration and incubated for different times (3, 12, 24, 48 h). The oil red staining was used to mark the intracellular lipid droplets and morphological changes. The cell culture supernatants were collected to test triglyceride content. Results: L-02 high fat cell model was feasible. High concentration of flavonoids (100 μmol/L) showed the significant decrease of lipid droplets and lower triglyceride levels. The triglyceride decreased with prolonged incubation time. Conclusion: Flavonoids from Coreopsis tinctoria can reduce triglyceride in the liver cells with fat degeneration. There is no significant liver cell enzyme damage. The effect depends on the concentration and incubation time.

Key words： Coreopsis tinctoria flavonoid triglyceride fat degeneration

Received: 02 November 2015

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	FANG Xuan
	LI Yali
	CHEN Xinmei

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I10/720

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