急性单核细胞白血病特异性miRNA分子标志物的鉴定

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Abstract Objective: To characterize the molecular markers of microRNA (miRNA) with specifically high expression in acute monocytic leukemia. Methods: A batch of miRNAs was firstly screened out for their significantly higher expression in THP-1 (acute monocytic leukemia cell line) by analyzing high-throughput miRNA transcriptome sequencing data of THP-1 and K562 (chronic myeloid leukemia cell line). These miRNAs were further tested in the THP-1 and K562 cell line by quantitative real-time PCR (qRT-PCR) to obtain the specifically high expressed miRNAs in THP-1 cell line. Finally, these screened miNRAs were verified in bone marrow samples of acute monocytic leukemia patients by qRT-PCR. The finally verified miRNAs could serve the candidates for clinical diagnosis of this disease in the future. Results: Three miRNAs that are specifically highly expressed in acute monocytic leukemia including let-7d-5p, miR-221-3p and miR-222-3p were characteried. Conclusion: By integrating the bioinformatics analysis and verification in cell lines and patient bone marrow samples, we finally characterized 3 miRNAs as potential molecular diagnostic markers in acute monocytic leukemia.

Key words： acute monocytic leukemia microRNA quantitative real-time PCR miRNA-Seq

Received: 26 February 2016

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	LIU Jinli
	LIU Shuge
	XIONG Qian
	HAN Li
	LI Wei
	WANG Wanheng
	ZHANG Zhaojun
	LI Quanzhen

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I10/703

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