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| Effects of microRNA-212 on migration and invasion of gastric cancer cell lines SGC7901 |
| YING Jianghui1, JIANG Peipei2, JIN Cancan2, PANG Wenyang2, CAI Yiqi2, ZHU Guanbao2. |
1.Department of Burn Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Gastroenterological Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
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| Cite this article: |
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YING Jianghui,JIANG Peipei,JIN Cancan, et al. Effects of microRNA-212 on migration and invasion of gastric cancer cell lines SGC7901[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(9): 644-648.
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Abstract Objective: To investigate the effect of human microRNA212 (miR-212) on the migration and invasion of gastric cancer cell line SGC7901. Methods: The i-miR-212 as the suppressor gene of miR-212 was transfected into SGC7901 cells according to different concentration (25, 50, 75, 100 nmol/L) via lipofectamin 2000. Empty vectors and unrelated fragment were also applied as controls. The expression of mature type miR-212 was detected by real-time PCR. Cell Counting Kit-8 was used to evaluate the effect of miR-212 on the proliferation of SGC-7901 cells, meanwhile Transwell assay for migration and invasion of SGC7901 cells. Results: Real-time PCR showed that cells Transfected with i-miR-212 had significantly low expression of miR-212, with the optimal concentration of miR-212 being 50 nmol/L; the expression of miR-212 was 18 times lower than empty group (P<0.01). The Cell Counting Kit-8 showed that the proliferation of cancer cells with i-miR-212 (50 nmol/L) was higher than that of the control group (P<0.01). The Transwells showed the migration and invasion of cancer cells with i-miR-212 (50 nmol/L) was higher than that of the control group (P<0.01), respectively. Conclusion: The i-miR-212 can effectively reduce miR-212 expression and promote the migration and invasion of SGC7901 cells. miR-212 may be a tumor suppressor gene in gastric cancer tumor cells.
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Received: 20 October 2015
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