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| The role of caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro |
| WANG Ruili1, DAI Wei1, DAI Yuanrong2. |
1.Department of Critical Care, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Respiratory Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
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WANG Ruili,DAI Wei,DAI Yuanrong. The role of caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(9): 629-632.
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Abstract Objective: To explore the role of caveolae/caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro. Methods: Recovery freeze-stored ASMCs of asthmatic rats were divided into five groups [group A (blank group), group R10 (roxithromycin 10 µg/mL), group R25 (roxithromycin 25 µg/mL), group R50 (roxithromycin 50 µg/mL) and group R100 (roxithromycin 100 µg/mL)]. The proliferation of ASMCs induced by roxithromycin were tested by CCK-8. The changes of caveolae in ASMCs were observed by transmission electron microscope. The protein expressions of caveolin-1 and P21 were measured by Western blot. Results: Roxithromycin could inhibit the proliferation of asthmatic rats ASMCs in vitro and had concentration-dependent manner. With the increased of intervention concentration, the expression of caveolae increased and the protein expressions of caveolin-1, P21 increased. Besides, compared with group A or group R10, the expressions of caveolin-1 and P21 protein in group R50 or R100 showed significantly different (P<0.05). There was significantly negative correlation between the cell activity rate of asthmatic rats and the expressions of caveolin-1 and P21 protein (r=－0.881, r=－0.951, P<0.05). The expression caveolin-1 was positively correlated with P21 (r=0.957, P<0.01). Conclusion: Roxithromycin inhibits the proliferation of ASMCs from asthmatic rats in vitro may partly through promoting the expression of P21 protein which induced by increased the expression of caveolae/ caveolin-1.
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Received: 23 October 2015
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