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| Effect of microparticles derived from bone marrow mesenchymal stem cells on cardiac fibroblasts |
| CHEN Mian1, WANG Jue2, GENG Zhimin3, PAN Lulu4, JIN Zengyou3, JIA Lianhong3, LU Jiacheng3, HUANG Niannian3, CHU Maoping4. |
1.Department of Paediatrics, Taizhou Hospital Affiliated to Wenzhou Medical University, Taizhou, 317000; 2.Department of Cardio-Thoracic Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 3.Department of Paediatrics, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 4.Children’s Heart Center, the Second Affiliated Hospital & Yuying Children’s Hospital, Institute of Cardiovascular Development and Translational Medicine, Wenzhou Medical University, Wenzhou, 325027
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| Cite this article: |
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CHEN Mian,WANG Jue,GENG Zhimin, et al. Effect of microparticles derived from bone marrow mesenchymal stem cells on cardiac fibroblasts[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(9): 625-628.
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Abstract Objective: To study the effects of microparticles derived from bone marrow mesenchymal stem cells (MSC-MPs) on proliferation, migration and collagen synthesis of cardiac fibroblast. Methods: MSCs were obtained from SD mice and cultured under the condition of serum free in hypoxia. Then the conditioned media were collected and microparticles were then isolated from the media. MSC-MPs were used to treat cardiac fibroblast. CCK-8 assay and cell scratch method were used to detect the effect of MSC-MPs on proliferation and migration of cardiac fibroblast, seperately. The expression of collagens I and III, α-SMA, vimentin and fibronectin in cardiac fibroblast were measured by means of qRT-PCR. Results: The proliferation ability of cardiac fibroblast was enhanced after treated with MSC-MPs. The migration ability was not significantly affected. The expression of collagens I and III, vimentin and fibronectin in cardiac fibroblast were promoted. Conclusion: MSC-MPs can enhance the proliferation ability and collagen synthesis of cardiac fibroblast to improve the cardiac function.
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Received: 10 March 2016
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