miR-187在肺癌组织中的表达及其效应

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Abstract Objective: To explore the expression of miR-187 in the tissues of lung cancer and its effect. Methods: By normalized to U6, the expressions of miR-187 in 32 lung cancer and matched non-tumor adjacent tissue specimens were examined by stem-loop real-time RT-qPCR method. The relationship between miR-187 expression and clinicopathological characteristics was further analyzed. After transfected with miR-187 mimics, the biological functions of miR-187 were determined by cell proliferation and cell cycle assay. Results: Expression of miR-187 in the tissue of lung cancer was obviously higher than that in non-tumor adjacent tissue specimens (t=5.236, P<0.0001). Clinical features correlation analysis showed that the down-expression of miR-187 was correlated with the pathological grading and the clinical staging (P<0.05). Functional studies indicated that the overexpression of miR-187 dramatically inhibited the proliferation of A549 cells in vitro and changed the situation of the cell cycle, arrested at G0/G1 phase. Conclusion: Down-expression of miR-187 in the patients of lung cancer may play a key role in the development and progression of lung cancer.

Key words： microRNA miR-187 lung neoplasms

Received: 19 August 2015

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	SUN Debin
	LAN Xiu

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I4/278

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