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| The effect of EGB on expression of TGFβ1, NF-κB, and FN in hepatic tissues from diabetic rats with non-alcoholic fatty liver disease |
| WANG Jiayuan1, FAN Jianshuang1, WU Liang2, HUANG Kate2, WANG Rongrong2 |
1.Pharmacy of Chinese Medicine, the Affiliated Wenzhou Chinese Medical Hospital of Zhejiang Chinese Medical University, Wenzhou, 325000; 2.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
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| Cite this article: |
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WANG Jiayuan,FAN Jianshuang,WU Liang, et al. The effect of EGB on expression of TGFβ1, NF-κB, and FN in hepatic tissues from diabetic rats with non-alcoholic fatty liver disease[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(4): 267-273.
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Abstract Objective: To observe the effect of extract of ginkgo biloba (EGB) on hepatic tissue from DM rats with non-alcoholic fatty liver disease (NAFLD). Methods: Thirty male Sprague-Dauley rats were divided randomly into three groups: normal control group, type 2 diabetic group and EGB-treated group. After being fed with high-fat feeding for 4 weeks, the later groups were injected with low dosage strepozotocin (30 mg/kg) intraperitoneally to induce NAFLD model of type 2 diabetes mellitus. The EGB treated group was gavaged with EGB at the dosage of 50 mg·kg-1·d-1 for 12 weeks. The pathologic changes of liver was observed under light microscopy (LM) stained with HE and VG staining respectively. Additionally, the protein expression of NF-κB, TGFβ1, FN, α-SMA were assayed by immunohistochemistry (IHC), the mRNA expression of TGFβ1, NF-κB, FN from rats livers were assayed by semi-quantitative RT-PCR. Results: Liver specimen from diabetic rats stained by HE and VG showed obvious liver fatty degeneration, ballooning degeneration, dotty necrosis necrosis with accompanying inflammatory infiltration and fibrosis. Meanwhile, we discovered that there were a great number of α-SMA reactive hepatic stellate cells in diabetic rats liver tissue. In addition, the protein expression of NF-κB, TGFβ1, FN (P<0.05, P<0.01, P<0.05) and the TGFβ1, FN mRNA expression (P<0.01, P<0.05) of diabetic rats livers were significantly higher than that of the normal control group. After treatment with EGB, the pathological change of liver and proliferation of hepatic stellate cell in diatetic rats relieved (P<0.05).Meanwhile, the protein expression of NF-κB, TGFβ1, FN (P<0.05) and the TGFβ1, FN mRNA expression (P<0.05) of diabetic rats livers decreased significantly compared with type 2 diabetic group. Conclusion: EGB can relieve NAFLD from diabetic rats. Down regulation of expression of NF-κB, TGFβ1, FN may be involved in it
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Received: 16 September 2015
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