亚慢性苯吸入暴露抑制小鼠单倍体精子核蛋白基因的表达

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Abstract Objective: To explore the major damage stage of spermatogenesis in mice submitted to subchronic inhalation of benzene. Methods: C57BL/6J mice were randomly divided into control, 1 ppm and 100 ppm benzene exposure group. Simulated occupational exposure condition, a subchronic inhalation exposure test was carried out using the dynamic control equipment. The pathological change of testicular tissue was observed with HE staining. The mRNA expression of marker genes of spermatogenesis was quantitatively tested with real-time polymerase chain reaction, the selected marker genes included Plzf of undifferentiated spermatogonia, Stra8 of differentiated spermatogonia, Sycp3 of primary spermatocyte, Tnp1 and Tnp2 of round spermatid, Akap4 of elongating spermatid, as well as Prm1 and Prm2. Results: Enhanced eosinophilic staining were observed in mice testicular tissue section of benzene exposure groups, a few karyolysis and exfoliative cells were found in 1 ppm exposure group, the phenomenon of mice testis germ cell degeneration and necrosis were more obvious in 100 ppm exposure group, the number of seminiferous tubules with no or few germ cells were increased obviously. It suggested that subchronic benzene exposure could induce pathological change in mice testis. The mRNA expression of Plzf, Stra8, Sycp3, Akap4 did not show statistical difference in all groups, Prm2 expression was significantly reduced in 1 ppm group than that of control group (P<0.01). The mRNA expression of transition protein Tnp1 and Tnp2, protamine Prm1 and Prm2 were significantly reduced in 100 ppm exposure group than that of the control and 1 ppm group. Conclusion: Subchronic benzene exposure can induce the pathological change in mice testis, down-regulate the mRNA expression of Tnps and Prms, mainly influence the process of histone replaced by protamine in round spermatid nuclei.

Key words： benzene sperm spermatogenesis transition protein protamine mice

Received: 17 September 2015

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	ZHANG Yaya
	QIN Qiongyu
	LIN Chong
	SUN Shuqiang
	ZHU Hua
	YANG Xinjun
	YANhongtao

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I4/249

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