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| Protective effect of ambroxol on experimental atelectasis |
| ZHANG Yili1, TU Junwei2, ZHOU Xiuyun3 |
1.Department of Critical-care Medicine, Jinhua Municipal Central Hospital, Jinhua, 321000; 2.Department of Respirology, Jinhua Municipal Central Hospital, Jinhua, 321000; 3.Department of Nephrology, Jinhua Municipal Central Hospital, Jinhua, 321000
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| Cite this article: |
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ZHANG Yili,TU Junwei,ZHOU Xiuyun. Protective effect of ambroxol on experimental atelectasis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(3): 187-189.
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Abstract Objective: To investigate the effect of ambroxol on experimental atelectasis. Methods: Eighteen male China experimental miniature pigs (CEMP) were randomly divided into three groups: contral group (C group), atelectasis group (M group) and ambroxol treatment group (T group), respectively (n=6). Ni-Ti alloys occluders were placed in the lower right main bronchus of M and T group animals with a bronchofiberscope after anesthesia. CEMP of T group were treated with high dose of ambroxol hydrochloride injection (30 mg/kg, q 8 h, respectively) after the occluders procedure was finished. The development of atelectasis was observed by computerized tomography scanning every three days after occluders were placed in. On the third day of atelectasis, bronchoalveolar lavage was carried out. The animals were killed at the end of experiment, dipalmitoylphosphatidylchol (DPPC), large aggregates (LA) and small aggregates (SA) from the bronchoalveolar lavage was measured using high performance liquid chromatography. SP-A from the bronchoalveolar lavage was measured using a sandwich ELISA technique. Histopathological changes of atelectasis were determined under a light microscope. Results: Typical pathology of atelectasis and computerized tomography imaging were observed in group M. Concentrations of DPPC, SP-A and rate of LA/SA in M group were significantly lower than that in C group, while concentrations of DPPC, SP-A and rate of LA/SA in T group were markly higer than those in M group. The area of atelectasis was shruken in T group compared to those in M group. Conclusion: These findings suggest that ambroxol has beneficial effect on experimental atelectasis. This may be the result that ambroxol can contribute to enhances pulmonary surfactant synthesis and reduce the conversion of LA to SA.
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Received: 03 September 2015
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[1] TOJO K, NAGAMINE Y, YAZAWA T, et al. Atelectasis causes alveolar hypoxia-induced inflammation during uneven mechanical ventilation in rats[J]. Intensive Care Med Exp, 2015, 3(1): 56-72.
[2] 涂军伟, 章义利, 王赛斌, 等. 小型猪可复性肺不张模型的建立[J]. 实验动物科学, 2015, 32(2): 23-26.
[3] STRANG C M, HACHENBERG T, FREDEN F, et al. Development of atelectasis and arterial to end-tidal PCO2-difference in a porcine model of pneumoperitoneum[J]. Br J Anaesth, 2009, 103(2): 298-303.
[4] ROBBINS SL, ANGELL M, KUMAR V. Basic pathology[M]. Washington: W.B. Saunders Com Pany, 1981: 374.
[5] CHRONEOS Z C, SEVER-CHRONEOS Z, SHEPHERD V L. Pulmonary surfactant: an immunological perspective[J]. Cell Physiol Biochem, 2010, 25(1): 13-26.
[6] HUANG W, MCCAIG L A, VELDHUIZEN R A, et al. Mechanisms responsible for surfactant changes in sepsis-induced lung injury [J]. Eur Respir J, 2005, 26(6): 1074-1079.
[7] MAGOMEdov M K, TITOVA G P, BARINOVA M V. Morphology of pulmonary atelectasis in patients operated on not operated on faking into account pulmonary surfactant state [J]. Arkh patol, 1979, 41(11): 57-64.
[8] 马玉腾, 田英平, 苏建玲, 等. 氨溴索对百草枯中毒大鼠肺组织损伤及肺表面活性蛋白-A的影响[J]. 中华劳动卫生职业病杂志, 2006, 24(6): 348-351.
[9] 付学明, 余加林, 刘官信, 等. 盐酸氨溴索和地塞米松对胎鼠肺表面活性蛋白基因表达的影响[J]. 中华儿科杂志, 2004, 42(6): 450-453.
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. [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2013, 43(9): 622-623. |
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