低频电刺激对低氧高二氧化碳大鼠比目鱼肌肌纤维类型的影响

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (2018 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To explore the effect of low frequency electrical stimulation on transition of rats’ soleus muscle fibre type after the long term chronic hypoxia-hypercapnia exposure. Methods: Forty male Sprague Dawley rats were randomly divided into four groups: the Normal Control (NC), Hypoxia-hypercapnia (HH), Hypoxia-hypercapnia+Tie-up (HT) and Low-frequency electrical stimulation (LFES). The HH, HT and LFES were placed in a chamber with hypoxia-hypercapnia for 8 h/d, 4 weeks in all. After 2 weeks, 30 min, 30 Hz, 1:1 cycle electrical stimulation was applied in the LEFS, this procedure lasted 2 weeks. The HT were only tied up and placed with electrode plates. Hematoxylin and Eeosin staining was used to observe pathological change of soleus muscle. Soleus fiber composition was visualized with immunochemistry, qRT-PCR was used to detect mRNA levels of MHC-I, MHC-IIa and IIx. Proteins expression of MHC-I, ERRγ, PPARβ was tested by Western Blot. Results: Increased inflammation cells and MHC-IIa/IIx mRNA, decreased cross section area, MHC-I, PPARβ protein and MHC-I mRNA were found in the HH and HT group compared with the NC. Compared with the HH and HT respectively, inflammation cells and MHC-IIa/IIx mRNA level were decreased, while mRNA level of MHC-I and protein expression of MHC-I, ERRγ and PPARβ were significantly increased after LFES. Conclusion: Hypoxia-hypercapnia leads to soleus muscle transition of MHC-I into MHC-IIa/IIx. However, LFES may reverse the muscle fiber transition induced by HH. PPARβ and ERRγ may have participates in this process.

Key words： hypoxia-hypercapnia electrical stimulation rats soleus myosin heavy chain

Received: 26 July 2015

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HUANG Shiyuan
	JIANG Xianxun
	SHEN Jie
	WANG Xiaotong.

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2016/V46/I3/157

[1] DECRAMER M, JANSSENS W, MIRAVITLLES M. Chronic obstructive pulmonary disease and comorbidities[J]. Lancet Respir Med, 2013, 1(1): 73-83.
[2] SWALLOW E B, REYES D, HOPKINSON N S, et al. Quadriceps strength predicts mortality in patients with moderate to severe chronic obstructive pulmonary disease[J]. Thorax, 2007, 62(2): 115-120.
[3] BARREIRO E, DE LA PUENTE B, MINGUELLA J, et al. Oxidative stress and respiratory muscle dysfunction in severe chronic obstructive pulmonary disease[J]. Am J Respir Crit Care Med, 2005, 171(10): 1116-1124.
[4] Koechlin C, Maltais F, Saey D, et al. Hypoxaemia enhances peripheral muscle oxidative stress in chronic obstructive pulmonary disease[J]. Thorax, 2005, 60(10): 834-841.
[5] PUENTE-MAESTU L, LAZARO A, HUMANES B. Metabolic derangements in COPD muscle dysfunction[J]. J Appl Physiol (1985), 2013, 114(9): 1282-1290.
[6] ELIASON G, ABDEL-HALIM S M, PIEHL-AULIN K, et al. Alterations in the muscle-to-capillary interface in patients with different degrees of chronic obstructive pulmonary disease[J]. Respir Res, 2010, 11(1): 97-101.
[7] BOURJEILY-HABR G, ROCHESTER C L, PALERMO F, et al. Randomised controlled trial of transcutaneous electrical muscle stimulation of the lower extremities in patients with chronic obstructive pulmonary disease[J]. Thorax, 2002, 57(12): 1045-1049.
[8] ABDELLAOUI A, PREFAUT C, GOUZI F, et al. Skeletal
muscle effects of electrostimulation after COPD exacerbation: a pilot study[J]. Eur Respir J, 2011, 38(4): 781-788.
[9] SILLEN M J, FRANSSEN F M, DELBRESSINE J M, et al. Efficacy of lower-limb muscle training modalities in severely dyspnoeic individuals with COPD and quadriceps muscle weakness: results from the DICES trial[J]. Thorax, 2014, 69  (6): 525-531.
[10] EL-KHOURY R, BRADFORD A, O’HALLORAN K D.Chronic hypobaric hypoxia increases isolated rat fast-twitch and slow-twitch limb muscle force and fatigue[J]. Physiol Res, 2012, 61(2): 195-201.
[11] 徐漫欢, 范小芳, 王小同, 等. 吸入低氧高二氧化碳对小鼠右心室收缩压及肺小动脉重建的影响[J]. 浙江医学, 2008, 30(1): 41-43.
[12] ARMSTRONG R B, PHELPS R O. Muscle fiber type composition of the rat hindlimb[J]. Am J Anat, 1984, 171(3):259-272.
[13] WAN Q, YEUNG S S, CHEUNG K K, et al. Optimizing electrical stimulation for promoting satellite cell prolifer-ation in muscle disuse atrophy[J]. Am J Phys Med Rehabil, 2016, 95(1): 28-38.
[14] LANGEN R C, GOSKER H R, REMELS A H, et al. Triggers and mechanisms of skeletal muscle wasting in chronic obstructive pulmonary disease[J]. Int J Biochem Cell Biol,  2013, 45(10): 2245-2256.
[15] OUDIJK E J, NIJHUIS E H, ZWANK M D, et al. Systemic  inflammation in COPD visualised by gene profiling in peripheral blood neutrophils[J]. Thorax, 2005, 60(7): 538-544.
[16] MANN C J, PERDIGUERO E, KHARRAZ Y, et al. Aberrant repair and fibrosis development in skeletal muscle[J]. Skelet Muscle, 2011, 1(1): 21.
[17] MUSCARITOLI M, ANKER S D, ARGILES J, et al. Consensus definition of sarcopenia, cachexia and pre-cachexia: Joint document elaborated by special Interest Groups (SIG) “cachexia-anorexia in chronic wasting diseases” and “nutrition in geriatrics”[J]. Clin Nutr, 2010, 29(2): 154-159.
[18] SHRIKRISHNA D, PATEL M, TANNER R J, et al. Quadriceps wasting and physical inactivity in patients with COPD [J]. Eur Respir J, 2012, 40(5): 1115-1122.
[19] 包绍智, 房春燕, 王小同, 等. 慢性低氧高二氧化碳对小鼠骨骼肌形态的影响[J]. 温州医学院院报, 2009, 39(1): 8-10.
[20] DE THEIJE C C, LANGEN R C, LAMERS W H, et al. Differential sensitivity of oxidative and glycolytic muscles to hypoxia-induced muscle atrophy[J]. J Appl Physiol, 2014,118(2): 200-211.
[21] SLOT I G, SCHOLS A M, VOSSE B A, et al. Hypoxia differentially regulates muscle oxidative fiber type and metabolism in a HIF-1alpha-dependent manner[J]. Cell Signal, 2014, 26(9): 1837-1845.
[22] GAN Z, RUMSEY J, HAZEN B C, et al. Nuclear receptor/microRNA circuitry links muscle fiber type to energy metabolism[J]. J Clin Invest, 2013, 123(6): 2564-2575.
[23] NARKAR V A, FAN W, DOWNES M, et al. Exercise and PGC-1alpha-independent synchronization of type I muscle metabolism and vasculature by ERRgamma[J]. Cell Metab, 2011, 13(3): 283-293.
[24] MATSAKS A, YADAV V, LORCA S, et al. Revascularization of ischemic skeletal muscle by estrogen-related receptor-γ[J]. Circ Res, 2012, 110(8): 1087-1096.