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| Remodeling of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation |
| JIN Peifeng1, ZHANG Hao2, LU Di1, WENG Jiakan1, JIANG Sheng1, SUN Chengchao1. |
1.Department of Thoracic and Cardiovascular Surgery, the First Affliliated Hospital of Wenzhou Medical University,Wenzhou, 325015; 2.Fu Wai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing, 100037
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| Cite this article: |
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JIN Peifeng,ZHANG Hao,LU Di, et al. Remodeling of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(1): 13-18.
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Abstract Objective: To investigated the feasibility of remodeling nervous system regeneration after myocardial infarction and the efficacy by which it may prevent ventricular arrhythmia following Schwann cells transplantation. Methods: Myocardial infarction was induced by left anterior descending artery ligation in syngenic Lewis rats, then the rats were randomized into cell transplantation group (n=31) and control group (n=30).Schwann cell were isolated from sciatic nerves, and immunohistochemical of S100 was carried out for the identification of Schwann cells. 5×106 Schwann cell were intramyocardially injected into the border zone of infarct region. The density of sympathetic and parasympathetic nerves and growth-associated protein 43 in the infarct region were stained by immunohistochemical method and immunofluorescence at 2 weeks after Schwann cells transplantation. Meanwhile, dynamic electrocardiography and programmed electric stimulation were also performed to detect the susceptibility of ventricular arrhythmias. Results: More than 95% of cells were S-100-positive Schwann cells during the entire culture process. Immunohistochemical and immunofluorescence staining illustrated increases in sympathetic and parasympathetic nerves in both groups. However, Schwann cells transplantation significantly increased the parasympathetic/sympathetic ratio at 2 weeks after cell injection. In addition,the Schwann cells also significantly decreased the low-/high-frequency ratio and susceptibility of programmed electric stimulation-induced ventricular arrhythmia at 2 weeks after cell injection. Conclusion: Transplanted Schwann cells in the infarcted myocardium can alter the ratio of parasympathetic/sympathetic nerve density to normalize irritable myocardium.
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