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| Determination of DEHP in white spirit by ultra performance liquid chromatography |
1.Department of Pharmacy, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035
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| Cite this article: |
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ZHOU Xuan1,WANG Zhe1,LI Junwei2, et al. Determination of DEHP in white spirit by ultra performance liquid chromatography[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(11): 811-.
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Abstract Objective: To establish a method to determine the concentration of di (2-ethylhexyl) phte (DEHP) in white spirit and study the different concentration between the same brand and the different brands. Methods: DEHP was detected in a solid phase extraction-ultra performance liquid chromatography (SPE-UPLC) method. DEHP was extracted from white spirit, using solid phase extraction column, then detected with ultra high performance liquid chromatograph. The mobile phase was acetonitrile-water and the flow rate was 0.4 mL/min. The UV detection wavelength was 224 nm. Results: The linear relation was good in this method, the RSD was less than 15%, and the recovery was between 90.08% and 103.32%. The low limit of the detection was 1 μg/mL. The samples with five different bland were all contain DEHP. Conclusion: The method is compeletely suitable for determination of DEHP in white spirit.
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Received: 16 February 2015
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