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| The construction of SW480 cells nude model transfected by hNIS gene and radionuclide imaging |
1.Department of Nuclear Medical, Wenzhou Medical University, Wenzhou, 325035; 2.Depaertment of Nuclear Medical, SIR RUN RUN SHAW Hospital, Zhejiang University, Hangzhou, 310020; 3.Radiological Department, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
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ZHANG Hongzheng1,DU Fan2,ZHOU Bailin3, et al. The construction of SW480 cells nude model transfected by hNIS gene and radionuclide imaging[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(11): 803-.
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Abstract Objective: To examine the feasibity of radionuclide imaging mediated by colon cancer cells (SW480) nude model transfected with a recombinant pcDNA3.1+-hNIS plasmid. Methods: pcDNA3.1+-hNIS was confirmed by restriction enzyme digestion, the hNIS gene and protein expression were detected after transfection, the SW480 cells of stably expressing hNIS were screened, the tumors nude mice model was established, then radioactive nuclide 99mTcO4- imaging was obtained. Results: After SW480 cells was transfected with pcDNA3.1+-hNIS, the hNIS gene and prote in expression were successfully detected. Radionuclide imaging was successfully mediated by colon cancer cells nude model. Conclusion: The hNIS expression can be achieved in colon nude model by the recombinant plasmid pcDNA3.1+-hNIS, and make the radionuclide imaging and radionuclide therapy of colon cancer as possible.
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Received: 26 March 2015
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