猪小肠黏膜下层与嗅鞘细胞生物相容性实验

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Abstract Objective: To explore the biocompatibility of small intestinal submucosa as a biological scaffold on the proliferation of olfactory ensheathing cells. Methods: The porcine small intestinal submucosa (SIS) was made according to Abraham’s methods. seeding the OECs on the porcine SIS for experimental group (SIS+OECs), OECs cultivating alone as control group. Then OECs’ growth, adhesion and proliferation of OECs on SIS was examined by HE stained and scanning electron microscope at different time during the cultivation, and the proliferation of OECs was examined with CCK8 as well. Results: OECs could attach to SIS within 24 hours after seeded on the SIS. After being cultured 3 to 5 days, OECs were intensive on the edge of SIS, well adhered to it and the cells were most fusiform and tripolar in shape. The combination of SIS and OECs was very excellently examinated by scanning electron microscope and Haematoxylin and eosin stained. And CCK8 measurement also revealed that, the quantity of OECs reached a stable level at 7 to 9 day and this level would not decreased until 11 d. Compared with these in contrast group, the proliferation of OECs on SIS had obvious difference during the former 5 days (P<0.05) but there was no significant difference after 7 day (P>0.05). Conclusion: Porcine SIS has good biocompatibility with rat OECs, although there is no evidence showing SIS promoting OECs’ proliferation. SIS can expectedly be used in repair of spinal cord injury as a scaffold where OECs may grow and adhere.

Key words： olfactory ensheathing cells small submucosa intestinal scaffold spinal cord injury

Received: 31 July 2014

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	LI Yu’an1
	LI Jia2
	XU Huazi1
	LIN Damu1
	WU Zhipeng1.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I9/656

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