柚皮素对RSV A2株感染引起气道黏液高分泌的抑制作用

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Abstract Objective: To investigate the expression levels of MUC5AC protein and mRNAin mucous hypersecretion model induced by respiratory syncytial virus (RSV), and explore the effect of Naringenin (Nar) on mucous hypersecretion induced by RSV. Methods: Human lung adenocarcinoma epithelial cells (A549) were subcultured, infected with RSV and treated with Nar 30-100 μmol/L. After it was treated for 24 hrs, the expression of MUC5AC at mRNA and protein level in the groups were determined by real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA). The morphological changes of MUC5AC protein were observed with immunofluorescence technology. Results: The expressions of MUC5AC protein and mRNA in all RSV infected groups were significantly higher than that in group C in a dose-dependent manners (all P<0.05). Nar of 30 and 100 μmol/Lsignificantly and dose-dependently decreased RSV-induced secretion of MUC5AC protein in cell supernatant and expression of MUC5AC mRNA (P<0.05), but still higher than that in group C (P<0.05). We also observed that RSV increased MUC5AC protein expression in the cytoplasm of A549 by immunofluorescence technology, and the protein expression decreased after pretreatment with Nar. Conclusion: Naringenin can inhibite the RSV-induced mucous hypersecretion of A549 cell.

Key words： Naringenin RSV mucous hypersecretion MUC5AC

Received: 18 March 2015

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	MENG Shanshan1
	2
	LV Fangfang1
	HU Xiaoguang1
	ZHANG Hailin1
	LI Changchong1

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I9/631

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