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The diagnostic value of human papilloma virus E6/E7 mRNA detection in CINII+ |
1.Department of Obstetrics and Gynecology, the Third Clinical College of Wenzhou Medical University, Wenzhou People’s Hospital, Wenzhou, 325000; 2.Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027 |
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Cite this article: |
HUANG Lingxiao1,PAN Qionghui1,ZHENG Jianqiong1, et al. The diagnostic value of human papilloma virus E6/E7 mRNA detection in CINII+[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(8): 583-.
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Abstract Objective: To evaluate the application value of detecting human papilloma virus (HPV) E6/E7 mRNA as a diagnose test for CINII+. Methods: From January to October in 2014, a total of 426 gynecological outpatients, whose TCT≥ASCUS or/and high-risk HPV DNA positive, were found by cervical lesions screening in Wenzhou People’s Hospital. All those outpatients performed HPV E6/E7 mRNA detection and histopathological examination. According to different histological results, samples were divided into 5 groups, the correlation between the expression of HPV E6/E7 mRNA and the severity of cervical lesions were analyzed. The ROC curve was used to assess the predictive value of HPV E6/E7 mRNA for CINII+ of ASCUS. Results: The cervical cancer group had the highest level of HPV E6/E7 mRNA while the chronic cervical inflammation group had the lowest. Spearman rank analysis demonstrated that the severity of cervical lesions was proportional to the expression of HPV E6/E7 mRNA (r=0.549, P<0.001). For CINII+, there was no significant difference between HPV E6/E7 mRNA and high-risk HPV DNA in positive rate (x2=0.197, P>0.05). The ROC curve showed that HPV E6/E7 mRNA>147.965 were superior to any other standards to scan CINII+. The sensitivity and negative predictive value of HPV E6/E7 mRNA and HR-HPV DNA in predicting CINII+ of patients with ASCUS showed no statistically significant difference (P>0.05), while the specificity and positive predictive value showed statistically significant difference (P<0.05). Conclusion: The copy number of HPV E6/E7 mRNA transcripts is correlated with severe degree of epithelial cervical dysplasia. The specificity and positive predictive value of HPV E6/E7 mRNA detection are higher than those of HPV DNA for diagnosis of CINII+ in patients with ASCUS, therefore HPV E6/E7 mRNA detection may be a valuable tool for triage and patient follow-up.
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Received: 01 February 2015
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