|
|
|
| Neruoprotective effect of bFGF on ischemic-reperfusion injury in SD rats via intranasal administration |
| 1.School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035; 2.Medical Insurance Office, Beijing Geriatric Hospital, Beijing, 100095 |
|
| Cite this article: |
|
JIANG Yina1,TIAN Furong1,LIN Qian1, et al. Neruoprotective effect of bFGF on ischemic-reperfusion injury in SD rats via intranasal administration[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(8): 560-.
|
|
|
|
Abstract Objective: To investigate the neuroprotective effects of bFGF (basic fibroblast growth factor) via intranasal administration for ischemic neurons of brain. Methods: The transient focal cerebral ischemia-reperfusion model of adult male SD rats was established with a method of line-plugging to the external carotid artery. Twenty-four hours after the reperfusion, behavioral test (neurologic deficit scoring) was carried out to evaluate the neurological deficiency. The neuroprotective effects of bFGF via intranasal administration to the model rats were evaluated with neurologic deficit score, infarct volume, histology and immunohistochemistry staining. Results: The transient focal cerebral ischemia-reperfusion model were established successfully. Comparing with the ischemia-reperfusion group, the level of infarction volume was significantly decreased (P<0.05), the number of nerve cells were increased and the expression of p-Akt positive cells were significantly up-regulated via intranasal administration of bFGF group. Conclusion: By the new pathway of nasal administration, bFGF can decrease the infarction volume and protect neurons from cerebral ischemic injury.
|
|
Received: 22 December 2014
|
| |
|
|
|
[1] 马宁, 蔡继福, 姜卫剑. 局灶性脑缺血动物模型的制备[J]. 神经损伤与功能重建, 2010, 5(1): 53-55.
[2] Noda M, Takii K, Parajuli B, et al. FGF-2 released from degenerating neurons exerts microglial-induced neuroprotection via FGFR3-ERK signaling pathway[J]. J Neuroinflammation, 2014, 11(76): 1176-1186.
[3] Enevoldsen MN, Kochoyan A, Jurgenson M, et al. Neuroprotective and memory enhancing properties of a dual agonist of the FGF receptor and NCAM[J]. Neurobiol Dis,2012, 48(3): 533-545.
[4] Zechel S, Werner S, Unsicker K, et al. Expression and functions of fibroblast growth factor 2(FGF-2) in hippocampal formation[J]. Neuroscientist, 2010 16(4): 357-373.
[5] Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1): 84-91.
[6] 程发峰, 宋少婷, 郭少英, 等. 神经功能损伤评分在大鼠脑缺血实验中的应用[J]. 中国比较医学杂志, 2011, 21(3): 43-48.
[7] 王桥生, 符晖, 曾红科. 线栓法大鼠局灶脑缺血模型的改进与评价[J]. 中南医学科学杂志, 2012, 40(3): 291-294.
[8] 陈茉弦, 敖丽娟, 李琦, 等. 脑卒中动物模型的建立与比较[J]. 中国康复医学杂志, 2011, 26(8): 779-782.
[9] Holland JP, Sydserff SG, Taylor WA, et al. Rat models of middle cerebral artery ischemia[J]. Stroke, 1993, 24(9):1423-1424.
[10] 于奎营, 韩江全, 张海霞. bFGF对大鼠脑缺血再灌注侧皮质区细胞凋亡及p-Akt(Ser473)表达的影响[J]. 广东医学, 2010, 31(23): 3035-3037.
[11] 韩江全, 于奎营, 何敏, 等. 葛根素对大鼠脑缺血再灌注侧皮质区细胞凋亡及p-Akt(Ser473)表达的影响[J]. 中国中西医结合杂志, 2012, 32(8): 1069-1072.
[12] 邢雪松, 吕威力, 赵海, 等. bFGF对局灶性脑缺血大鼠脑组织GRP78表达的影响[J]. 解剖学研究, 2012, 34(4): 290-292.
[13] 袁凤刚, 郝艳玲, 张春平, 等. MK801对大鼠全脑缺血/再灌 注ASK1信号通路影响以及神经元的保护[J]. 中国药理学通报, 2014, 30(2): 179-185.
[14] 朱红霞. 经鼻腔给药靶向中枢神经系统的研究进展[J]. 中国医药指南, 2012, 109(23): 105-106.
|
|
|
|
