双抗体靶向阿霉素纳米药物的研制及其靶向抗骨肉瘤作用

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Abstract Objective: To synthesize double antibody complex-glycol chitosan doxorubicin nanoparticles (Abc-GC-Dox) and study its targeting effect of osteosarcoma in mice. Methods: Abc-GC-Dox and GC-Dox were prepared by emulsion polymerization method, in vitro immunofluorescence was used to study the intake test of HER2+carried MG-63 cells in Abc-GC-Dox and GC-Dox. Fourteen mice with osteosarcoma were randomly divided into 2 groups. After corresponding treatment, different drugs (Abc-GC-Dox and GC-Dox) were given via tailvein, and the targeting effect was observed. Results: Stable Abc-GC-Dox and the quantity of drug • loading and entrapment were gotten. Compare to GC-Dox, there was a better drug concentration in osteosarcoma in mice, which showed a well targeting effect in vivo. Conclusion: Abc-GC-Dox is successfully developed and the nanoparticles demonstrated targeting aggregating distribution. In future, Abc-GC-Dox can be a potential drug for the clinical treatment of tumors.

Key words： double antibody complex doxorubicin ethylene glycol chitosan nanoparticle targeted therapy osteosarcoma nude mice

Received: 06 January 2015

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	ZHENG Yijing
	ZHANG Xiaolei
	LIN Yan
	WU Yaosen
	ZHANG Qiong

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I8/547

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