EIF5A2在结直肠癌细胞中的表达及在5-Fu化疗抵抗中的作用

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Abstract Objective: To research EIF-5A2 overexpression in colorectal cancer and its effect on chemotherapy. Methods: EIF5A2 expression in colorectal carcinoma (CRC) line (LOVO, SW48 and DLD1) was measured by Western-blot. LOVO cells were cultured in 5-Fu for 48 hours, and EIF5A2 expression was measured by Western-blot. LOVO cells were treated with EIF5A2-siRNA and exposed to 0.2 μg/L 5-Fu for 48 hours. Cell toxicity was assayed by MTT. Results: EIF5A2 was expressed in all three different CRC and was highest in LOVO cells (P<0.05). 5-Fu treatment led to elevated EIF5A2 expression (P<0.05). The transfection of EIF5A2-siRNA could improve the inhibit rate of LOVO to 5-Fu (P<0.05). Conclusion: EIF5A2 is expressed in CRC lines and it can enhance the chemoresistance of 5-Fu.

Key words： colorectal neoplasm EIF5A2 5-Fu chemoresistance

Received: 07 August 2014

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	FAN Yongshen1
	CHANG Yinjuan2.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I7/516

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