沉默黏着斑激酶表达抑制大肠癌转移的体内研究

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Abstract Objective: Focal adhesion kinase gene silencing by Short Hairpin RNA can effectively inhibit colorectal cancer’s metastasis were confirmed through injection of FAK-knockdown colorectal cancer cells into nude mice. Methods: Twenty-four nude mice were randomly divided into three groups which were experimental group which inject FAK-knockdown colon cancer cells, negative control group whick inject negative control colon cancer cells and untreated group which inject untreated colon cancer cells. Nude mice were sacrificed after 6 weeks and tumor formation rate, mortality, tumor growth, liver and lung’s metastasis, miscrometastasis were observed and FAK protein expression were determined with HE staining and immunohistochemical staining. Results: Tumor formation rate was 100% in each group. Distant metastases, mortality and FAK protein expression of experimental group was significantly lower than that of the other two groups (P<0.05), meanwhile, the malignant lesion extent was lighter. While negative control group and untreated group were not significantly different (P>0.05). Conclusion: Lentiviral packaging shRNA-mediated FAK gene silencing can significantly inhibit liver and lung metastasis of colorectal cancer. FAK is expected to be a new target for treatment of colon cancer metastasis.

Key words： focal adhesion kinase SW620 colorectal neoplasms nude mouse metastasis

Received: 18 February 2014

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	HONG Weiwen1
	SU Guoqiang2
	YING Hongan3
	LIN Feng1
	LIANG Weidong1.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I7/497

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