Notch 1信号通路蛋白在不同程度颅脑损伤大鼠中的表达

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Abstract Objective: By detecting Notch 1 signaling pathway proteins Jagged 1 and Hes 1 in light of heavy colsed craniocerebral injury. The role of Notch 1 signaling pathways in traumatic brain injury was explore and the new direction of treatment for closed craniocerebral injury was provide. Methods: Use lateral fluid percussion method to make the light, medium and heavy closed craniocerebral trauma models. Groups of rats after 24 h of neurologic deficits were evaluated through the nervous system disease severity score (NSS), then use immunohistochemistry and Western blot methods were used to detect Notch 1 signaling pathway downstream factors Jagged 1 and Hes 1. Results: The NSS score difference among the TBI groups was statistically significant (P<0.05), the brain contusion was heavier, the neurologic deficits were more obvious, which means the model was established successfully. Immunohistochemistry and Western blot showed that in brain contusion the expression of Hes 1 and Jagged 1 levels were significantly higher than that the control group (P<0.05), and increased with the degree of brain injury, the expression of the proteins was increased. Conclusion: Notch 1 signaling pathway may be involved in the development process of craniocerebral injury, and may become the new target for therapy after traumatic brain injury.

Key words： traumatic brain injury lateral fluid percussion Notch 1 signaling pathway

Received: 17 March 2015

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	Articles by authors
	WANG Wei
	GAO Zhichao
	ZHU Penglei
	ZHENG Weiming
	TU Ming.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I7/488

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