一种特异性检测汞离子的大肠杆菌的构建

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Abstract Objective: To construct a specific biosensor to detect mercury ions using the enhanced cyan fluorescent protein as a reporter. Methods: The fusion reporter vector, merR-O\P-ecfp-T, was constructed using ecfp as reporter gene. Then the reporter vector merR-O\P-ecfp-T was transformed into Escherichia coli MC4100 wide type strain. The growth conditions optimization of this completed biosensor and its specificity and the optimum detection range parameters were determined. Results: The results showed that the mercury biosensor had a good specificity, the optimal detection range of mercury concentration was 0.15-38.4 μmol/L. A specific biosensor were successfully constructed to detect mercury ions. Conlusion: The work provides the foundation for developing other hypersensitive biosensors for the chemicals such as explosives, polycyclic aromatic hydrocarbons, polychlorinated hydrocarbons and heavy metals that are important to national security, human health, environment, energy supply and food safety.

Key words： enhanced cyan fluorescent protein mercury ion detection E.coli

Received: 23 March 2015

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	XIAO Fanglan
	LV Panpan
	YAN Xijuan
	JI Songjun
	WANG Wu
	LV Jianxin.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I7/474

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