兔急性灭多威中毒毒代动力学观察

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Abstract Objective: To observe the toxicokinetics of acute methomyl poisoning in rabbits, and to explore its damaging effects on tissue and organ. Methods: Disposable gavageing methomyl dose 12 mg/kg, to observed symptoms and signs at different time points. The process of metabolism and absorption of methomyl were detected by Liquid chromatography-mass spectrometry/mass spectrometry in rabbit plasma, drawing concentration-time curve. The toxicokinetic parameters were analyzed and calculated by DAS 2.0 software. The pathological changes of brain, heart, liver, kidney were observed under inverted fluorescence microscope. Blood biochemistry was analyzed with biochemical analyzer at each time point. Results: The parameters were AUC0-t (1 209.734± 219.542) μg/L·h. MRT0-t (1.731±0.144) h, t1/2z (1.471±0.277) h, Tmax 0.25 h, CLz/F (9.576±1.816) L·h-1·kg-1, Vz/F (20.225±4.703) L/kg in methomyl toxicokinetics. The pathological changes, cellular infiltration and edema were visible under light microscope in heart, liver, brain. Compared with previous exposure, the Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK) elevated after exposure for 6 h, the differences were statistically significant (P<0.05). Troponin-I (cTn-I) increased at 2 h after exposure, the differences were statistically significant (P<0.05). Cholinesterase (AchE) activity decreased rapidly after 0.25 h exposure, the differences were statistically significant (P<0.05), returned to normal levels after exposure for 6 h. Conclusion: The toxicokinetics of Methomyl gavaged poison is in accordance with two-compartment model, its half-life in vivo is short, metabolism is rapid after poisoning, different degrees damages are detected in the brain, heart, liver, kidney organs.

Key words： methomyl toxicokinetics liquid chromatography mass spectrometry pathological changes rabbits

Received: 23 January 2015

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	WANG Zhiyi1
	WANG Chunlong2
	WANG Xianqin3
	CHEN Jin1
	SUN Laifang1
	MA Jianshe2

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I7/469

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