大鼠心肌组织提取物抑制缺氧和无血清诱导的大鼠骨髓间充质干细胞凋亡

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Abstract Objective: To explore tissue extracts from myocardium of rats protects bone marrow stromal cells (BMSC) against hypoxia/serum deprivation (SD)-induced apoptosis, and investigate its potential mechanism. Methods: The BMSC were isolated from female SD rat and cultured in vitro. The ischemic microenvironment was simulated by hypoxia/SD and tissue extracts from myocardium of rats were extracted by ultracentrifugation. Cell counting kit was used after 24 h hypoxia/SD treatment to observe the cell number of BMSC and to select the proper concentration. The inverted phase contrast microscope observation and heochst 33258 nucleus staining were used to detect the morphological changes of the cells. Western blot was used to detect the expression of caspase-3. Results: The result of cell counting kit showed that with the extracts concentration increasing, the cell number increased. The proper concentration of myocardium tissue extracts was 100μg/mL. Compared with the apoptotic model (AM) group, there were less apoptotic cells in myocardium tissue extracts group and control group. The expression level of cleaved caspase-3 detected was higher in AM group than that in the NMTE and control group. Conclusion: Tissue extracts from myocardium of rats protect BMSC against hypoxia/SD-induced apoptosis. The protective effect is significant when the extracts concentration reached 100μg/mL. The protective effect is related to caspase-3 signal pathway.

Key words： bone marrow stromal cells,tissue extract,cell apoptosis,caspase-3,rats

Received: 26 August 2013

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	LU Di
	MAO Chenxi
	JIN Peifeng
	WANG Jue
	SUN Chengchao.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I1/7

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