人线粒体DNA缺失宫颈癌细胞及其转线粒体细胞的建立与初步分析

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Abstract Objective: To generate mtDNA-depleted Hela S3 cells and the transmitochondrial cybrids and investigate mtDNA replication and mitochondrial structure. Methods: Hela S3 cells were incubated with 100 ng/mL ethidium bromide (EtBr). Polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) were performed in ρ0 Hela S3 cells. Platelet-mediated transmitochondrial cybrids were established on the basis of ρ0 Hela S3 cells and identified by PCR, qPCR and transmission electron microscopy (TEM). Results: ρ0 Hela S3 cells had a low level of mtDNA replication and distorted cristae of mitochondria, whereas transmotichondrial cybrids had a normal mtDNA replication and regular arranged cristae of mitochondria. Conlusion: The generation of transmitochondrial cybrids provides a viable method for the further research of the effect of mtDNA mutations on mitochondrial respiratory function associated with diseases.

Key words： mtDNA-depleted cells transmitochondrial cybrids mtDNA copy numbers cristae of mitochondria

Received: 07 March 2015

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	Articles by authors
	WEN Chaowei
	YE Wei
	ZHOU Huaibin
	LV Jianxin
	LI Wei.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I6/402

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