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| Effects of extract of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2 in myocardial cells induced by high glucose and its underlying mechanism |
Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
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LI Jianmin,WU Ling,ZHANG Di, et al. Effects of extract of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2 in myocardial cells induced by high glucose and its underlying mechanism[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(6): 397-.
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Abstract Objective: To investigate the effects of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2, Bax, Bcl-2 and Caspase-3 in mitochondrial apoptotic pathway in neonatal rat myocardial cells induced by high glucose and underlying mechanism. Methods: Left ventricule myocardial cells of neonatal SD rats were isolated and cultured with low-glucose culture media (LG group), low-glucose culture media added EGB (LG +EGB group), high-glucose culture media (HG group) and high-glucose culture media added EGB (HG+EGB group) in vitro respectively. The ratio of apoptosis of myocardial cells was analyzed by flow cytometry. The expression of Drp-1, Mfn-2, Bax, Bcl-2 and Caspase-3 were detected with western blot and/or RT-PCR respectively. Results: Compared to the LG group, the rate of apoptosis, the expression of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2 in ventricule myocardial cells were increased in HG group significantly (P<0.05), while the expression of Mfn-2 and Bcl-2 were decreased significantly (P<0.05). Compared to HG group, the rate of apoptosis, the expression of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2 in HG+EGB group were decreased significantly (P<0.05), while the expression of Bcl-2 was increased significantly (P<0.05). But the expression of Mfn-2 had no significant difference (P>0.05). Conclusion: EGB plays an important role in attenuation cardiomyocyte apoptosis induced by high glucose via its upregulation the expression of Bcl-2 and down regulation of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2. It may be one of the underlying mechanisms for EGB to treat diabetic cardiomyopathy.
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Received: 15 February 2015
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