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| Effect of Sedum sarmentosum Bunge Extract on TGF-β1-induced renal epithelial-to-mesenchymal transition and matrix accumulation |
| 1.Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Wenzhou Key Laboratory of Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015 |
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Abstract Objective: To investigate the protective effects of Sedum sarmentosum Bunge Extract (SSBE) on TGF-β1-induced epithelial-to-mesenchymal transition (EMT) and matrix accumulation in renal tubular epithelial cells (NRK-52E). Methods: NRK-52E cells were divided randomly into: control group treated with only solvent, TGF-β1 group treated with TGF-β1 at the concentrations with 5 µg/L and SSBE group treated with TGF-β1 plus SSBE at the concentrations with 10 and 100 mg/L. The morphology of the NRK-52E cells was observed under inverted/phase contrast microscope. Immunofluorescent analysis was performed to detect the expression of epithelial marker α-smooth muscle actin (α-SMA), mesenchymal marker E-cadherin, and matrix component type III collagen. Gene expression of α-SMA, bone morphogenic protein-7 (BMP-7), tight junction protein-1 (Tjp1), Col1a1, Col3a1, MMP-2, and TIMP-2 were also quantified by real-time RT-PCR. Results: In TGF-β-treated NRK-52E cells, fibrosis-like phonotype was obviously increased. TGF-β1 increased the expression of α-SMA, and decreased the expression of E-cadherin and Tjp1. Also, TGF-β1 enhanced the expression of type I and III collagens. Treatment with SSBE at the concentrations with 10 and 100 mg/L inhibited TGF-β1-induced fibrosis-like phenotype of NRK-52E cells, accompanied with down-regulated expression of α-SMA, Col1a1 and Col1a1 and up-regulated expression of E-cadherin and Tip1. In addition, SSBE increased the expression of BMP-7 and the ratio between MMP-2 and TIMP-2. Conclusion: SSBE treatment reduce TGF-β1-induced fibrosis-like reaction in renal tubular epithelial cells through inhibiting EMT and matrix accumulation.
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Received: 11 September 2014
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