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| Adjusting and controlling effect of microRNA-494 on extracellular matrix of secretion cells in renal interstitial fibroblast |
Department of Nephrology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
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LIN Haixia,SU Zhen,SHU Danhua, et al. Adjusting and controlling effect of microRNA-494 on extracellular matrix of secretion cells in renal interstitial fibroblast[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(3): 162-.
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Abstract Objective: To explore the adjusting and controlling effect of microRNA-494 (miR-494) on extracellular matrix of the secretion cells in renal interstitial fibroblast (NRK-49F) cells after stimulation of TGF-β1. Methods: NRK-49F cells were cultured in vitro and divided into: TGF-β1 (3 ng/mL) group, miR-494 mimics (50 ng/mL) group, miR-494 inhibitor (50 ng/mL) group, co-treated with miR-494 mimics (50 ng/mL) and TGF-β1 (3 ng/mL) group, co-treated with miR-494 inhibitor (50 ng/mL) and TGF-β1 (3 ng/mL) group, blank control group. The expression of miR-494, collagen I (Col I), fibronectin (FN) mRNAs were analyzed by stem-loop real-time quantitative PCR (RT-qPCR) techniques. The expression of Col I protein was analyzed by Western Blot. Results: Compare with blank control group, the expression of miR-494 (P<0.01), Col I (P<0.05) and FN (P<0.01) mRNA were significantly increased in TGF-β1 group, the expression of Col I and FN mRNA were significantly increased in miR-494 mimics group (P<0.05), the expression of Col I (P<0.05) and FN (P<0.01) mRNAs were significantly decreased in miR-494 inhibitor group. Compared with TGF-β1 group, the expression of Col I and FN mRNAs were significantly increased in co-treated with miR-494 mimics and TGF-β1 group (P<0.05), the expression of Col I and FN mRNA were significantly decreased in co-treated with miR-494 inhibitor and TGF-β1 group (P<0.05), the expression of Col I protein was consistent with Col I mRNA (P<0.05). Conclusion: miR-494 can promote the TGF-β1 inducing secretion and deposition of extracellular matrix.
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Received: 08 May 2014
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