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| Genetic polymorphism of nine short tandem repeat loci in han ethnic population in Wenzhou |
| 1.Experiment Center of Basic Medicine, Wenzhou Medical University, Wenzhou, 325035; 2.Teaching Center of Biology Experiment of Wenzhou Medical University, Wenzhou, 325035; 3.Judicial Identification Center of Wenzhou Medical University, Wenzhou, 325035 |
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WU Shuzhen1,3,ZHANG Hongqin2, et al. Genetic polymorphism of nine short tandem repeat loci in han ethnic population in Wenzhou[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(2): 123-.
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Abstract Objective: To analyze the alleles and genotype frequencies of nine short tandem repeats loci, those are D18S1364, D12S391, D13S325, D6S1043, D2S1172, D11S2368, D22-GATA198B05, D8S1132, D7S3048, of Han Ethnic Population in Wenzhou. Methods: extract DNA respectively from anticoagulant of 355 unrelated individual samples of Han Ethnic population living in Wenzhou. Deal the nine STR loci of the samples with multiple PCR amplification by STR_Typer_10_v1 kit. Analyze the PCR products by 310 genetic analyzer and GeneMapper ID 3.2v software of AB company. Deal the results with statistical analysis on the allele frequency and common forensic parameters by PowerState V12.xls software. Results: 15, 12, 9, 17, 15, 13, 11, 10 and 12 alleles were observed respectively from the nine STR loci. The distribution of genotype frequencies matched the Hardy-Weinberg equilibrium, P was more than 0.05. The heterozygote was more than 0.7831. The polymorphism information content was more than 0.7666. The combined power of discrimination was more than 0.9266. Conclusion: All of the nine STR loci of Han Ethnic Population in Wenzhou in this study have high genetic polymorphism, and it can be used as good genetic markers. The data obtained in this study can be used in individual identification, paternity test and population genetics investigation of Han Ethnic population living in Wenzhou.
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Received: 10 March 2014
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2015, Vol. 45 
