2型糖尿病小鼠小脑代谢物的1H NMR研究

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Abstract Objective: To investigate the diabetes-related metabolic changes in the cerebellum of db/db mice. Methods: Specimens of the cerebellum were dissected from the 17-week db/db mice and the age-matched controls. The cerebellum tissue samples were extracted and analyzed by 1H NMR-based metabonomics. Results: Compared to the controls, the db/db mice exhibited markedly different metabolic profiles, which included significant decrease in the levels of glycine (P<0.01), aspartate (P<0.01), glutamate (P<0.01), GABA (P<0.01), N-acetyl-aspartate (P<0.01) and alanine (P<0.05) and increase in the levels of glutamine (P<0.01), taurine (P<0.05) and lactate (P<0.01). Conclusion: These metabolic changes suggest that glucose metabolism and amino acid metabolism in the cerebellum of db/db mice are disordered, which provides clues for further understanding the pathogenesis of diabetic encephalopathy.

Key words： metabonomics diabetes, type 2 metabolite NMR db/db mice

Received: 08 October 2014

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	Articles by authors
	WEI Tingting
	ZHENG Yongquan
	WANG Hui
	XIA Huanhuan
	XU Cuicui
	YE Xinjian
	ZHAO Liangcai
	GAO Hongchang

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2015/V45/I2/119

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