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| Intervene of Hedgehog signal on expression of proliferating cell nuclear antigen in renal tubular epithelial cells |
| 1.Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Wenzhou Key Laboratory of Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015 |
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| Cite this article: |
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LU Hong1,WU Lianfeng1,LIN Chengcheng2, et al. Intervene of Hedgehog signal on expression of proliferating cell nuclear antigen in renal tubular epithelial cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2015, 45(1): 14-.
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Abstract Objective: To explore and investigate the activation and inhibition of Hedgehog signal regulated on expression of proliferating cell nuclear antigen (PCNA) in renal tubular epithelial cells (NRK-52E). Methods: NRK-52E cells were randomly divided into control group (treated with solvent only), activated group (treated with Shh at the concentrations with 10 μg/L) and treatment group (treated with Shh at the concentration of 10 μg/L plus cyclopamine at the concentrations with 5 μmol/L). After cultured for 24 h, immunofluorescence staining was used to evaluate the expressions of Ptch1, Smo, Gli1, and PCNA. The expression levels of Ptch1 and Smo mRNA were quantified by real-time RT-PCR. Results: NRK-52E cells without drug treatment maintain slowed proliferation state, and HH signal was not activated, and PCNA was in low expression. Treatment with exogenous recombinant protein Shh for 24 h, the mRNA or protein expression levels of Smo and Gli1 were significantly increased, and the expression of Ptch1 was decreased, suggesting that Shh activates the HH signal (P<0.05). In this process, the expression of PCNA protein was significantly increased. NRK-52E cells showed proliferation, and morphologic change occurred. After cyclopamine intervention, the activity of HH signal was decreased (P<0.05), and PCNA expression was also inhibited. In addition, abnormal proliferation of NRK-52E cells was inhibited, and increased apoptosis and necrosis cells. Conclusion: HH signal is involved in the proliferation of renal tubular epithelial cell. Regulating the activation of HH signal influences PCNA expression and cell proliferation.
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Received: 16 April 2014
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