|
|
|
| Regulatory effect of metformin on oxidative stress response in mouse hepatic ischemia/reperfusion injury |
| WANG Xinyu1, XU Junpeng1, WANG Xiaoting1, LIU Xiujie1, HUANG Qinghao2, CHEN Sian3, WANG Wantie1. |
| 1.Institute of Ischemia/Reperfusion Injury, Wenzhou Medical University, Wenzhou 325035, China;2.Emergency Department of Liuzhou People’s Hospital, Liuzhou 545000, China; 3.The First School of Medicine,School of Information and Engineering, Wenzhou Medical University, Wenzhou 325035, China |
|
| Cite this article: |
|
WANG Xinyu,XU Junpeng,WANG Xiaoting, et al. Regulatory effect of metformin on oxidative stress response in mouse hepatic ischemia/reperfusion injury[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2024, 54(1): 47-52.
|
|
|
|
Abstract Objective: To explore the effect of metformin on oxidative stress response in hepatic ischemia/reperfusion injury (HI/RI) in mouse. Methods: Fifteen SPF grade male C67BL/6 mice were randomly divided into three groups with 5 mice in each: the control group, the hepatic ischemia/reperfusion injury group (HI/RI group) and the hepatic ischemia/reperfusion injury+metformin group (MET group). The morphology of liver tissue was observed under the ordinary light microscopy; the degree of liver damage was evaluated with HE staining and PAS stain; the levels of ALT, AST, malondialdehyde (MDA) and Glutathione (GSH) were determined with a chemical kit. Results: Compared with the control group, the overall morphology, HE staining, and PAS staining of the HI/RI group showed an increase in the degree of liver tissue damage; The concentrations of ALT and AST in serum had a significant increase (P<0.01), so did the level of MAD in liver tissue (P<0.01), while the level of GSH significantly decreased (P<0.01); The red fluorescence intensity results in DHE staining showed a cumulative increase in ROS. Compared with the HI/RI group, the gross morphology, HE staining, and PAS staining of MET showed a significant reduction in liver tissue damage; The levels of ALT and AST in serum were significantly reduced (P<0.01), so did the MDA level in liver tissue (P<0.01), while the GSH level significantly increased (P<0.05); The DHE results showed a decrease in ROS accumulation. Conclusion: Metformin alleviates liver injury caused by ischemia/reperfusion, and it inhibits the oxidative stress response induced by liver ischemia/reperfusion.
|
|
Received: 16 May 2023
|
| |
|
|
|
|
|
|
