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| Experimental research of the hNIS transfected SW480 cells mediated radioiodine therapy |
Department of Nuclear Medicine, Wenzhou Medical University, Wenzhou, 325035
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DU Fan,ZHOU Bailin,ZHANG Hongzheng, et al. Experimental research of the hNIS transfected SW480 cells mediated radioiodine therapy[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(5): 334-.
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Abstract Objective: To detect the killing effect of colon cancer cells (SW480) by iodine 131 (131I). Methods: Cell apoptosis was detected when taken in 131I in vitro test and perchlorate suppression experiments and AnnexinV-FITC/PI double dye flow cytometry instrument. Results: Stably-hNIS-gene-expressing cell line was about 8 times higher than the control group and blank plasmid group in 24 h iodine uptake capacity. Perchlorate inhibition tests showed that the iodine uptake of hNIS-SW480 was the hNIS gene’s function. AnnexinV-FITC/PI double dye flow cytometry showed apoptosis index in the transfection group was higher than that of the control group and blank plasmid group. There were significant differences that radioactive iodine SW480 cells transfected with better killing ability under 100 μCi 131I in 24 h. Conclusion: 131I has significant cell killing effect on SW480 cells transfected hNIS.
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Received: 26 September 2013
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2014, Vol. 44 
