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| Correlation between human cytomegalovirus infection and systemic lupus erythematosus |
1.Department of Rheumatology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Nephrology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 3.The First Clinical Medical College of Wenzhou Medical University, Wenzhou, 325035; 4.Department of Microbiology and Immunology, Wenzhou Medical University, Wenzhou, 325035
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| Cite this article: |
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CHEN Jing1,ZHANG Huidi2,LOU Weiyang3, et al. Correlation between human cytomegalovirus infection and systemic lupus erythematosus[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(5): 318-.
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Abstract Objective: To analyze infective status of the human cytomegalovirus (HCMV) in systemic lupus erythematosus (SLE) patients and explore its relevance to clinical indicators of SLE. Methods: Peripheral blood leukocytes (PBLs) and serum samples were collected and separated from 60 patients with SLE and 111 healthy individuals. Highly sensitive and specific PCR method was used to investigate HCMV-UL55 gene and UL138 gene in PBLs, whereas Roche’s electrochemiluminescence detection kit was used to investigate the HCMV-specific serum IgG and IgM. The positive rates and correlation between UL55, UL138 genes detection in PBLs and sera IgG, IgM in SLE patients were explored in SLE patients and healthy individuals. The relationship between cellular HCMV infection in PBLs and common clinical indicators of SLE was further analyzed. Results: Agarose gel electrophoresis and sequencing analysis showed established PCR could be used to detect HCMV-UL55 gene and UL138 gene. HCMV IgG in serum and HCMV UL138 gene detection in PBLs were almost all positive, there was no significant difference between SLE patients and healthy individuals (P>0.05). On the contrary, the positive rate of HCMV serum IgM test was low; the positive rate of SLE patients (6.67%) was slightly higher than that of the healthy individuals (0.90%) (P=0.052); While the HCMV infection rate by detecting HCMV-UL55 gene in PBLs was significantly higher in patients with SLE than that in the healthy control (P<0.01). Analyzing the correlation among the four detection methods of HCMV infection, the HCMV-UL138 gene detection in PBLs had a good consistency with HCMV-specific serum IgG test. Analysis of HCMV infection in PBLs and clinical indicators of SLE patients, unlike HCMV-UL138 gene, a number of clinical indicators were significantly higher in the SLE patients with HCMV-UL55-positive PBLs than that in the SLE patients with HCMV-UL55-nagtive PBLs. Conclusion: There is a certain correlation between HCMV infection and SLE, but the results of different detection methods are significantly different.
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Received: 27 November 2013
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. [J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2022, 52(8): 0-. |
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2014, Vol. 44 
